Recent development of DNA markers provides powerful tools for population genetic analyses. Amplified fragment length polymorphism (AFLP) markers result from a polymerase chain reaction (PCR)-based DNA fingerprinting technique that can detect multiple restriction fragments in a single polyacrylamide gel, and...
PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples. jejuni and also strains of that species which lack detectable hippuricase activity. The third PCR assay, based on the sequence of a cloned (putative) ... ...
Cluster analysis, based on both the virulence phenotypes and RFLP patterns, indicates that P. teres possesses a high degree of diversity at the species and subspecies levels. The high degree of polymorphism revealed by ND218 will make this probe a useful tool for the DNA fingerprinting of P. ...
(T-RFLP)is one of microbial population fingerprinting methods that is based on labeled primers-PCR to identify microbial composition according to different terminal restriction fragment lengths.The applications and technical optimizations were overviewed of T-RFLP through the procedures of DNA preparation...
Genetic variations among 17 accessions of zoysiagrasses collected from natural populations in Japan were investigated by RFLP analyses of chloroplast DNA (cpDNA) and nuclear DNA. These accessions were classified into five species based on morphological characteristics: Zoysia japonica, Z. matrella, Z....
What two enzymes are needed to produce recombinant DNA? Enzymes are usually proteins. Based on what you know about how proteins are made, how would a cell make more of a particular enzyme in order to increase the concentration of that particular enzyme? What is the...
(1990) Potential applications of map-based cloning to plant pathology. Physiol. Mol. Plant Pathol. 37: 81–94. Article CAS Google Scholar Zhao, X.P. and Kochert, G. (1992) Characterization and genetic mapping of a short, highly repeated, interspersed DNA sequence from rice (Oryza sativa...
Except for the RAPD markers, all the markers correlated genetic distance with single cross performance and heterosis which showed that they could be useful in predicting single cross performance and heterosis in intrapopulation crosses for broad-based populations. Our results indicate that AFLP seemed ...
These fragments were repeatable across DNA samples extracted from different trees of the same accession or extracted at different times, and across separate PCR runs. Seventeen unique marker phenotypes were identified. The 48 trifoliate orange accessions were classified into four major groups based on ...
Functional integrated genetic linkage map based on EST-markers for a sugarcane (Saccharum spp.) commercial cross The growing availability of ESTs provides a potentially valuable source of new DNA markers. The authors examined the SUCEST database and developed EST-deri... KM Oliveira,LR Pinto,TG ...