In this study, we used two polymerase chain reaction (PCR)-based fingerprinting methods-amplified fragment length polymorphism (AFLP) and restriction fragment length polymorphism (RFLP) of laccase and manganese peroxidase genes-to assess genetic differences among strains and independently evolving lineages ...
Identification of aphid (Hemiptera: Aphididae) species of economic importance in Kenya using DNA barcodes and PCR-RFLP-based approach Aphids are among pests of economic importance throughout the world. Together with transmitting plant viruses, aphids are capable of inflicting severe crop ... G ...
The clinical applicability of genomic HLA class II typing techniques has increased after the introduction of PCR-based typing strategies. In typing by PCR ... H Zetterquist,O Olerup - 《Human Immunology》 被引量: 267发表: 1992年 Susceptibility to develop celiac disease is primarily associated wit...
Fingerprinting method for phylogenetic classification and indentification of microorganisms based on variation in 16S rRNA gene sequences[J]. Biotechniq- ues, 2000, 29(1):108-115. [20]Moritz C. Applications of mitochondrial DNA analysis in conserva- tion :a critical review[J]. Molecular Ecology...
Rapid discrimination and classification of the Lactobacillus plantarum group based on a partial dnaK sequence and DNA fingerprinting techniques The Lactobacillus plantarum group comprises five very closely related species. Some species of this group are considered to be probiotic and widely applied... CH...
(T-RFLP)is one of microbial population fingerprinting methods that is based on labeled primers-PCR to identify microbial composition according to different terminal restriction fragment lengths.The applications and technical optimizations were overviewed of T-RFLP through the procedures of DNA preparation...
These fragments were repeatable across DNA samples extracted from different trees of the same accession or extracted at different times, and across separate PCR runs. Seventeen unique marker phenotypes were identified. The 48 trifoliate orange accessions were classified into four major groups based on ...
PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori. DNA sequence diversity among 60 independent isolates of the gastric pathogen Helicobacter pylori was assessed by testing for restriction fragment length po... N,Akopyanz,N,... - 《Nucleic Acids Research》...
Cluster analysis, based on both the virulence phenotypes and RFLP patterns, indicates that P. teres possesses a high degree of diversity at the species and subspecies levels. The high degree of polymorphism revealed by ND218 will make this probe a useful tool for the DNA fingerprinting of P. ...
Except for the RAPD markers, all the markers correlated genetic distance with single cross performance and heterosis which showed that they could be useful in predicting single cross performance and heterosis in intrapopulation crosses for broad-based populations. Our results indicate that AFLP seemed ...