最终结果如图所示，红色曲线代表对照组，绿色部分表示活化的T细胞。每次增殖后，CFSE荧光强度会减少一半。这就是CFSE T细胞增殖实验的全过程，是不是非常简便呢？
CFSE kit来自于thermo, 以下为实验步骤: CFSE assay For new CFSE tube, add 18 ul DMSO into the tube, to get 5 mM (working solution 1:1,000 dilute) 1 mL complete RPMI for 10e6 cell; 0.5 ml for < 5e6 cell 1.1 ul 5mM in 110 ul PBS In tube bottom, put 1ml cells In tube top, ...
东大生物的方法可以评估测试品在由CD3 / CD28抗体诱导下的T细胞活化过程中的细胞增殖作用,测试方式包括CFSE标记稀释法和细胞内IFN-γ水平将通过流式细胞仪(FCM)测试法。 测试程序和方法 代表性结果 T cell response in mouse CD4+T and CD8+T cell activation/proliferation assay for 2 days as measured by CFSE...
T cellproliferation in response to stimulation by a peptide–MHC complex can be measured by (1) the incorporation of the radioactive nucleotide tritiatedthymidineinto the DNA of dividing but not resting cells or (2) thedilutionof afluorescent dye,carboxyfluoresceinsuccinimidyl ester (CFSE), that dec...
CFSECell divisionIL-12IL-15IL-18IL-2LymphocyteNK cellProliferationT cellLymphocytes proliferate in response to several stimuli. In many situations, a rapid lymphocyte expansion, or the identification of a slow dividing cell subpopulation may be of great interest. Thus, it is necessary to perform ...
Proliferation Index PI 7 9112 545 186 909 6823 03 本实验结果也表明 T细胞激活增殖用淋巴细 胞表面分子的荧光单抗染色 经流式细胞仪上测定 可反映不同增殖时间的T细胞亚群的比例 图1 但并不能提供各个亚群更为详细的增殖动力学变 化 而用CFSE标记法 结合测定淋巴细胞CD分 子 则可以反映出PHA和CD3 mAb体外...
T cell sub-population proliferation monitored by CFSE labelling in response to in vitro re-stimulation with H1N1 influenza.Eric, A. LefevreB., Veronica CarrCharlotte, F. InmanHelen, PrenticeIan, H. BrownSharon, M. BrookesFanny, GarconMichelle, L. Hill...
结论 以CFSE标记淋巴细胞,结合流式细胞术可有效检测出不同T细胞亚群对不同刺激剂的增殖反应以及增殖 动力学变化。 [关键词]羧基荧光素乙酰乙酸琥珀酰亚胺酯;增殖;T细胞亚群;流式细胞术 [中图分类号]R392.12 [文献标识码]A DeterminationoftheproliferationofCFSElabeledhumanTlymphocytesubpopulationsbyflow cytometry ...
now showing the T-cell sub-population with different CFSE staining intensities, by which the proliferation rate of the T-cells can be specifically analyzed. This video demonstrates the protocol for CFSE staining of mouse splenocytes, which are stimulated with an anti-CD3 antibody. This is follo...
883? A 韪 瓣 巷 B CFSE A 未刺激组B 刺激组 图1PHA 激活PBMC 后各T 细胞亚群的扩增比例 AUnstimulatedgroupBStimulatedgroup Fig.1ProportionofexpandedTcellsubsetsfromPBMCstimulatedwithPHA 2CelIQuest 软件分析 取数据采用CellQuest 软件分析,通过前向角和 侧向角圈定淋巴细胞,通过直方图确定CFSE 的荧光 强度,...