Adoptive transfer of chimeric antigen receptor (CAR)-engineered T cells combined with checkpoint inhibition may prevent T cell exhaustion and improve clinical outcomes. However, the approach is limited by cumulative costs and toxicities. To overcome this
VCN was determined for all DNAs via digital droplet PCR (ddPCR; BioRad), as described in ref. 61, and using the primer/probe sets (Integrated DNA Technology) specific for the retroviral vectors (for murine CAR-T cell assessment) or specific for the lentiviral vector (for human CAR-T cell...
To precisely determine if a low abundance of TET2mut existed before the 2nd_CRS, ddPCR was performed and revealed a small TET2mut clone already existed in PBMCs at pre-treatment indeed and mainly concentrated in CD3 positive host T cells (Fig. 6j, Supplementary Fig. 10c). This variation ...
Therefore, we analyzed three different organ sites (bone marrow, lymph nodes, and spleen) for the presence of CA HIV-1 DNA by droplet digital Polymerase Chain Reaction (ddPCR), either at the end of cART treatment or corresponding time points for the non-treated animals (see experimental ...
sds for three independent experiments using cells coming from three different hiv controllers are shown; statistics were calculated by unpaired t test. ∗ p < 0.05. (d) for each cell contact, the actin signal was analyzed and quantified with imaris software in 3d. two representative experiments...
Four days later, T cells were collected and used for total DNA preparation. The intact HIV-1 proviruses were determined by intact proviral DNA assay (IPDA) using a droplet digital polymerase chain reaction (ddPCR) system (Bio-Rad). IPDA is a novel quantitative approach for measuring the ...
In patients with advanced non-small cell lung cancer (NSCLC), droplet digital PCR (ddPCR) is increasingly used to genotype... C Silveira,AC Sousa,A Janeiro,... - 《Translational Lung Cancer Research》 被引量: 0发表: 2021年 iTRAQ technology combined 2DLC-MS /MS differentially expressed ...
Samples were used immediately for ddPCR (40 cycles; Bio-Rad) and run as 4–6 technical replicates. The raw spectral data were analysed using the Bio-Rad software to plot the signal for mutant and wild-type alleles. All runs contained positive and negative controls. Results were analysed in ...
Percentage of hCD45+CD33+cells in the BM and spleen at terminal analysis 15 d after T cell infusion. Numbers were adjusted for hCD3+T cells.e, Number of FLT3D835Y-mutated BM hCD45+CD3−cells determined by ddPCR.f,g, Number of mouse (m)CD45+cells in BM (f) and mTCR-β+CD...
‘off-the-shelf’ CAR T cells, but the in vitro differentiation of TiPS often yields T cells with suboptimal features. Here we show that the premature expression of the T-cell receptor (TCR) or a constitutively expressed CAR in TiPS promotes the acquisition of an innate phenotype, which can...