值得振奋的是,近期全球知名《Blood》杂志公布了“首个非基因编辑的CD5.CAR-T细胞胞临床试验(NCT0308190)的惊艳数据”,主要针对复发/难治性(r/r)、CD5阳性的成熟T细胞淋巴瘤(TCL)患者,包括外周T细胞淋巴瘤(PTCL)、血管免疫母细胞性...
gridExtra (v2.3),tidyverse (1.3.2),gtable (v0.3.2),scales (v1.2.1),lubridate (v1.9.2),survminer (0.4.9),survcomp (1.40.0),RColorBrewer (v1.1.3),GGally (v2.1.2),ggforce (v0.4.1),TCellExTRECT (v1.0.1),MatchIt (v4.5.0) 和 dNdScv (v0.0.1.0)。
Basar R, Daher M, Rezvani K.Next-generation cell therapies: the emerging role of CAR-NK cells.Blood Advances, 2020, DOI: 10.1182/bloodadvances.2020002547. Neelapu SS, Locke FL, Bartlett NL, et al.Axicabtagene ciloleucel CAR T-cell therapy in refractory large B-cell lymphoma.New England...
首先将人工制备的T淋巴细胞进行磁珠分选纯化,将2.5×108个外周血单个核细胞(peripheral blood mononuclear cell, PBMC)重悬后按照人总T细胞分选试剂盒说明书进行分选纯化。将收集到的纯化后细胞用流式细胞术进行纯度验证。样品中加入CD-PE/CD3-FITC/CD45-PerCP混合抗体...
The immunogenicity of HVV vaccinations was evaluated by measuring Gag-specific CD8+and CD4+T cell responses in blood 1 week after each vaccination, the peak time point as found in our previous study22, as well as at a later time point (week 76) to assess durability of response. Gag-CM9 ...
Fig. 1: Glutarate is an endogenous regulator of CD8+T cell function. a,b, Percentage of CD62LhiCD44hi(a) and number of total murine CD8+T cells (b) after 7 days of treatment with 400 µM of test compound. Ordinary one-way analysis of variance (ANOVA) relative to untreated contr...
使用Human Regulatory T Cell Staining Kit进行流式检测。人抗凝血染色FSC/CD4 (左), CD25/大鼠IgG2a同型对照, PE (中)和CD25/FoxP3(右)。粘连细胞已被排除。实验在Thermo Fisher公司的Attune NxT流式细胞仪上进行。 试剂盒组分 组分 编号 25Test 100Test 抗人CD4, FITC (克隆号:RPA-T4) AH00401 150 μ...
患者群体特征:纳入的 283 例患者中位年龄 66 岁,弥漫性大 B 细胞淋巴瘤未另行分类(Diffuse Large B - cell Lymphoma Not Otherwise Specified)占比 77% 。CAR - T 治疗后,总体缓解率(Overall Response Rate,ORR)为 80%,完全缓解(Complete Response,CR)率为 64% 。2 年无进展生存期(Progression - Free Su...
《Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the expression of Gadd45b in cell cycle pathway》 作者:Zhongrui Yan, Fengyi Guo, Qing Yuan, Yu Shao, Yedan Zhang, Huiyan Wang, Shaohua Hao and Xue...