Hosono, M, Boer, OJ, Wal, AC (2003) Increased expression of T cell activation markers (CD25, CD26, CD40L and CD69) in atherectomy specimens of patients with unstable angina and acute myocardial infarction. Atherosclerosis 168: pp. 73-80...
We have, therefore, expanded our earlier work by using a panel of different T cell activation markers (CD25, CD26, CD40L, CD69). The study is based on 58 culprit lesions from patients who underwent coronary atherectomy. There were four groups of patients: chronic stable angina ( n=13)...
HER2-XPAT protein and unmasked HER2-TCE promoted similar T-cell activation in the tumor microenvironment (TME), indicated by increased expression of activation marker CD25 on both helper and cytotoxic T cells (Fig. 3c). T cells were not activated in peripheral blood where human HER2 is not ...
Cloning of mouse Ox40: a T cell activation marker that may mediate T-B cell interactions. A cDNA library was prepared from the murine Th cell line Th2 D.10 and used to clone the murine homologue of Ox40 by polymerase chain reaction. Comparison o... DM Calderhead,JE Buhlmann,AJVD Eert...
Here, we show immunosuppressive property of streptavidin on T cell activation and proliferation. Upon CD3 and CD28 stimulation, CD4+ T cells produce interleukin 2 (IL-2) and express IL-2 receptor α chain (CD25). Addition of streptavidin in T cell culture suppressed IL-2 synthesis and CD25 ...
The inhibition was not cytokine mediated, was dependent on cell contact between the regulatory cells and the responders, and required activation of the suppressors via the TCR. Inhibition could be overcome by the addition to the cultures of IL-2 or anti-CD28, suggesting that the CD4+CD25+ ...
CellTrace dyesCell division Anti-CD25Activation marker Anti-CD134Activation marker Brdu or EdUDNA synthesis/proliferation 3H-ThymidineDNA synthesis/proliferation Cytokine profiling Treg cells have been shown to restrict T cell function through dive...
O. A dominant, coordinated T regulatory cell-IgA response to the intestinal microbiota. Proc. Natl Acad. Sci. USA 106, 19256–19261 (2009). Article CAS PubMed PubMed Central Google Scholar Maloy, K. J. et al. CD4+CD25+ TR cells suppress innate immune pathology through cytokine-dependent...
That’s why we developed our amazingly simple Buoyancy Activated Cell Sorting (BACSTM) technology. By using tiny glass microbubbles coated with streptavidin and marker-specific biotinylated antibodies, our products can gently and accurately isolate cells of interest. With no additional equipment needed ...
However, CD25 can be expressed by any activated T cell, so it cannot be used as a suppressor-cell marker after perturbation of the immune system. CD4+CD25+ T cells are potent suppressors of the activation of both CD4+ and CD8+ T cells in vitro. Suppression is mediated by a cell-...