Cancer cell killing by colony formation assay Target cells, including MSLN−/NCL+-231, MSLN−/NCL−-231, MSLN+/NCL+-231, MSLN+/NCL−-231, and MSLN−/NCL−-10A, were assayed at a density of 1 × 104 cells to evaluate their vulnerability to the cytotoxic effects of MSLN-...
Sustained expression of programmed cell death receptor-1 (PD-1) is correlated with the exhaustion of T cells, and blockade of the PD-1 pathway is an effective immunotherapeutic strategy for treating various cancers. However, response rates are limited, and many patients do not achieve durable res...
T cells are dynamically regulated immune cells that are implicated in a variety of diseases ranging from infection, cancer and autoimmunity. Recent advancements in sequencing methods have provided valuable insights in the transcriptional and epigenetic r
Downstream analysis, a vital step post-target identification, typically uses gene set enrichment analysis (GSEA) to identify functional pathways in T cell-mediated tumor cell cytotoxicity and validate key phenotypes of related functions. For instance, employing CRISPR/Cas9 for cellular engineering or sma...
T cell biology leading to significant advances in our understanding due to both the tremendous heterogeneity of T cells, as well as the presence of a small number of cells to have impacts on disease states. Finally, as primary cells from ex vivo states are often harder to work with, ...
Overexpression of glycolysis-related molecules impaired T cell killing of tumor cells, whereas inhibition of glycolysis enhanced T cell-mediated antitumor immunity in vitro and in vivo. Moreover, glycolysis-related gene expression was higher in melanoma tissues from ACT-refractory patients, and tumor ...
In hematopoietic cell transplants, alloreactive T cells mediate the graft-versus-leukemia (GVL) effect. However, leukemia relapse accounts for nearly half of deaths. Understanding GVL failure requires a system in which GVL-inducing T cells can be tracked
The success of cancer immunotherapy depends in part on the strength of antigen recognition by T cells. Here, we characterize the T cell receptor (TCR) functional (antigen sensitivity) and structural (monomeric pMHC-TCR off-rates) avidities of 371 CD8 T c
Targeting membrane proteins could improve the efficacy of T cell–based immunotherapies. To facilitate the identification of T cell targets, we developed a hybrid genetic screening system where the Sleeping Beauty (SB) transposon and single guide RNA cas
The recently identified G-protein-coupled receptor GPR171 and its ligand BigLEN are thought to regulate food uptake and anxiety. Though GPR171 is commonly used as a T cell signature gene in transcriptomic studies, its potential role in T cell immunity ha