Sustained expression of programmed cell death receptor-1 (PD-1) is correlated with the exhaustion of T cells, and blockade of the PD-1 pathway is an effective immunotherapeutic strategy for treating various can
Collectively, our data show that UTX promotes gene expression patterns that enhance cytotoxic T lymphocyte (CTL) killing but limit CD8+ T cell persistence during chronic infection. Results UTX is required for CD8+ T cell-mediated control of disseminated virus infection We assessed whether UTX ...
Downstream analysis, a vital step post-target identification, typically uses gene set enrichment analysis (GSEA) to identify functional pathways in T cell-mediated tumor cell cytotoxicity and validate key phenotypes of related functions. For instance, employing CRISPR/Cas9 for cellular engineering or sma...
Targeting membrane proteins could improve the efficacy of T cell–based immunotherapies. To facilitate the identification of T cell targets, we developed a hybrid genetic screening system where the Sleeping Beauty (SB) transposon and single guide RNA cas
(45), exosomes are nanovesicles between 30 and 120 nm in diameter and shed by a variety of different cell types, including those of hematological origin, such as B-cells, T-cells, dendritic cells, and non-hematological origin, such as epithelial cells, neuronal cells, and tumor-derived cells...
investigate the relationships between exhausted T cell signatures with other cell types within the tumor18. Specifically, cancer-associated fibroblasts (CAF) had specific gene signatures associated with T cell infiltration sug- gesting key interactions between these cell types. Furthermore, they identify...
The recently identified G-protein-coupled receptor GPR171 and its ligand BigLEN are thought to regulate food uptake and anxiety. Though GPR171 is commonly used as a T cell signature gene in transcriptomic studies, its potential role in T cell immunity ha
Patient's γδ T Cells Are Hyperreactive Against Malignant Cells To determine the patient's γδ T cells' cytotoxic properties, we conducted killing assays using Daudi, A549, and MDA- MB-436 tumor cell lines. The patient's PBMCs were able to induce significant lysis of all studied cell ...
Briefly, the lung sections were reviewed and evaluated for epithelial damage, peribronchovascular cell infiltrate, and interstitial-alveolar cell infiltrate. Epithelial damage was scored as: 0 (no damage); 1 (increased cytoplasm of epithelial cells without desquamation); 2 (epithelial desquamation ...
Viral mutations are well known to subvert the antiviral T cell-mediated response by impacting HLA binding, the Ag presentation machinery, and T cell receptor recognition. Here we show how naturally occurring EBV variants affect the flexibility and conformation of a T cell epitope, which subsequently...