To ensure consistency and to avoid inter-lab- oratory variation [11], reverse-sequence syphilis testing was conducted at Johns Hopkins University (JHU) and included a screening chemiluminescence antibody test (
Syphilis antibody was positive. Reflex rapid plasma reagin (RPR) was reactive, and the titer was 1:32. Lumbar puncture (LP) was performed on hospital day 8 and cerebrospinal fluid (CSF) venereal disease research laboratory (VDRL) was positive. The cell count was normal, protein was 72 mg...
(fluorescent treponemal antibody, treponema pallidum hemagglutination assay, enzyme immunoassay, Western blot), were obligatory. In recent decades, new methods were elaborated (e.g., BioPlex total screen, tests with β2-GPI-dependent anticardiolipin antibody, the ARCHITECT syphilis treponema pallidum ...
One fair-quality retrospective study (n = 139) evaluated the prozone phenomenon using rapid plasma reagin (RPR) testing.18 The prozone phenomenon occurs when undiluted serum containing a high titer of nonspecific antibody (as may occur in secondary syphilis) produces a false-negative result att...
RPR and VDRL can be used to monitor treatment response as they correlate fairly well with disease activity and typically decrease with treatment. Treponemal-specific tests include the EIA for antitreponemal IgG, the T. pallidum hemagglutination (TPHA) test, and the fluorescent treponemal antibody ...
Abbott laboratories, Wiesbaden, Germany, and Genscreen HIV1/2 version 2, Bio-Rad laboratories, California, USA, respectively) were performed in paral- lel, with Western blot (Bio-Rad Laboratories, California, USA) used as the tie-breaker for any discordant antibody results between the two assay...
is the presence of excess serum antibody which would interfere with the binding of antibody to bound antigen on the POCT strip. This is described as a hook effect, and is similar to the prozone phenomenon observed with non-treponemal flocculation tests such as the RPR assay [31]. Healthcare...