Synthetic Control Arms (SCAs) are a powerful tool in clinical trials. This is particularly true in cases where traditional control arms are difficult to implement. This could be due to ethical concerns, logistical challenges or limited patient availability. Either way, synthetic control arms can be...
Rethinking placebos: embracing synthetic control arms in clinical trials for rare tumorsExternal comparator arms should be used when investigating novel therapies for gastrointestinal stromal tumor and other rare tumors to facilitate drug testing and regulatory approvals....
Through the introduction of homology arms and spacers, it was possible to increase the circularization efficiency of mRNA with various lengths up to 5 kb (Wesselhoeft et al.2018). Circular mRNA showed a stable protein expression profile over a longer duration than that of linear mRNA, confirmin...
b, c H1299 cells were transfected with dCas9 (under the survivin promoter), MPH, and a high-performance activating sgRNA against IFNG (sgIFNG or a negative control sgNC). Cells were incubated for 48 h in the presence of 10 μg/ml of IgG or anti-IFNγ (Ab) and were then ...
In optimizing sequence-defined OAAs (see Section 2.5) for pDNA delivery, Petra Kos et al faced the dilemma between polyplex shielding and pDNA compaction. In their case, co-formulation of ligand-PEG-two-arm OAAs with PEG-free three-arms was critically required for polyplex stabilization and fu...
In practical use, avoid using two hands near the circuit and near the outputs, to avoid an accident where the shortest path for current could pass through the arms to the user's chest and heart. All electrical com- ponents should be kept away from or shielded from the liquids in the ...
These techniques rapidly increase RBP diversity at a pace unachievable through natural evolution and help to create synthetic phage populations one step ahead of their bacterial hosts in the co-evolutionary arms race. Dunne et al. combined RBP and lysogeny engineering to reprogram the temperate, ...
Cell lysates were centrifuged at 12,000 rpm for 5 mins and the supernatant were used for co-immunoprecipitation with an anti-AKT1 antibody, the mouse IgG as the negative control and Dynabeads™ Protein G Immunoprecipitation Kit (Invitrogen#10007D). The immunoprecipitates were subjected to western...
“protocells” and find the transition between the non-living and living matter by assembling three components: first, a metabolism for extracting energy from the environment and to construct, salvage and discard aged building blocks; second, an informational program like nucleic acids to control the...
In this paper, we use EHR data to construct synthetic control arms for treatment-only single arm trials. We propose a novel nonparametric Bayesian common atoms mixture model that allows us to find equivalent population strata in the EHR and the treatment arm and then resample the EHR data to ...