The synthesis of plasma proteins directed by mRNA from human brain tissues was studied by combining in vitro or in ovo translation of mRNAs with crossed immunoelectrophoresis of the mRNA-directed labeled polypeptides, followed by autoradiography of the washed plates. Poly(A)-containing mRNA was ...
A primed, constant infusion of the tracer L-[ring-(2)H(5)]phenylalanine was performed in colitis piglets after 14 d to determine the fractional synthesis rates of proteins in small intestinal mucosa, colon, and liver and of plasma proteins (total protein, fibrinogen, albumin). Colon ...
We then modified reversibly immobilized proteins with protein-reactive ATRP initiators and, after ATRP, we released and analyzed the protein polymers. The activity and stability of PARIS-synthesized and solution-synthesized conjugates demonstrated that PARIS was an effective, rapid, and simple method to...
Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen Analysis of the cell culture fluid from two new human hepatoma-derived cell lines reveals that 17 of the major human plasma proteins are synthesized and se... B Knowles,C Howe,D Aden - ...
Total plasma proteins. The rate of incorporation of phenylalanine tracer into the intravascular pools of total plasma proteins, albumin, and fibrinogen was linear (Fig. 1). FSR and ASR of the total plasma protein pool were higher in MRP than in MR piglets (Table 3; P = 0.05). However, ...
where FSR is the fractional synthesis rate of myofibrillar proteins, ΔMPEmyo is the change in enrichment of 2H in muscle protein-bound alanine, ΔMPEplasma is the change in enrichment of 2H in free alanine found in plasma and t is time. Statistics Depending on the number of the groups com...
Proteinsynthesis(initiation) Proteinsynthesis(elongation) Proteinsynthesis(termination) ProteinssynthesizedontheER: 1.Willbesecreted 2.Willtraveltolysosomes 3.Areincorporatedintothe plasmamembrane EndoplasmicReticulum ProteinTranslocation:ProteintranslationonandintotheEndoplasmicReticulum SignalsequencestargetaproteinforER...
The amino acid leucine stimulates protein synthesis in skeletal muscle of the neonate, but less is known about the effects of the leucine metabolite β-hydroxy-β-methylbutyrate (HMB). To determine the effects of HMB on protein synthesis and the regulation of translation initiation and degradation ...
The substrate was placed on the holder at a 20° angle of inclination to ensure successful deposition. Radiofrequency magnetron sputtering deposition was conducted with a silver target in magnetron discharge plasma at the sputtering power of 60 W for 5 min using a VTC-300 system (ZKDS ...
Thus, NANS-mediated synthesis of sialic acid is required for early brain development and skeletal growth. Normal sialylation of plasma proteins was observed in spite of NANS deficiency. Exploration of endogenous synthesis, nutritional absorption, and rescue pathways for sialic acid in different tissues...