The most common type of joint, the synovial joint, features a fluid-filled space between smooth cartilage pads at the end of articulating bones. Surrounding the joint is a capsule of tough dense irregular connective tissue lined with synovial membrane. The outer layer of capsule may extend into...
possesses three important properties: 1) It is a closed system that mimics the sealed environment of the synovial joint in vivo; 2) It contains de-nucleated fluid and 3) It reproduces both a cracking phenomenon and subsequent refractory period identical to that which is seen in real synovial ...
Synovial fluid is important for the preoperative etiological diagnosis of suspected periprosthetic joint infection (PJI) or septic arthritis (SA). GENECUBE, an automated real‐time PCR assay, was used to detect bacterial mecA (methicillin resistance) and was compared with microbiological culturesfor ...
The potential of five different commercial DNA isolation methods to remove real-time PCR inhibitors from the synovial fluid of horses, cattle and pigs was investigated. All kits with the exception of one included a silica column-based purification of the DNA. With the fifth kit, DNA purification...
Faryna A, Goldenberg K: Joint Fluid. In Clinical Methods: The History, Physical, and Laboratory Examinations. Edited by: Walker H, Hall W, Hurst J. Boston: Butterworths; 1990. Google Scholar da Silva MA, Arruda MA: Mechanization of the Bradford reaction for the spectrophotometric determination...
synovium- a thin membrane in synovial (freely moving) joints that lines the joint capsule and secretes synovial fluid synovial membrane tissue layer,membrane- a pliable sheet of tissue that covers or lines or connects the organs or cells of animals or plants ...
Blood was collected from the antecubital vein into heparinised tubes, whereas synovial fluid was collected by aseptic arthrocentesis from the knee joint. For ethical reasons, the investigators were instructed to collect a minimum amount of synovial fluid from the joint to allow the bioanalytical ...
Chemoattractant and cytokine protein and RNA levels in synovial and interfacial tissue and fluid were assessed by Luminex assay and real-time quantitative polymerase chain reaction. Cytokine production and degranulation by stimulated synovial tissue versus normal blood NK cells were evaluated by ...
(day 41), immunofluorescence staining of mouse knee joint synovium showed that the number of mast cells and T cells in the transfer group increased and T cells were infiltrated in the lining layer (Fig.4c). We also performed mast cell adoptive transfer in serum transfer induced arthritis (STA...
The healthy synovial lining layer consists of a single cell layer that regulates the transport between the joint cavity and the surrounding tissue. It has been suggested that abnormalities such as somatic mutations in the p53 tumor-suppressor gene contribute to synovial hyperplasia and invasion in rhe...