airtight, light-protected (foil wrappedor dark) polypropylene container in the refrigerator between uses.Under these conditions, working solutions will reliably last 6 or7 days.1.5 Reusing Working SolutionsWe have had varying reports about the number of times aworking solution of SYBR stain can be ...
Staining Minigels with SYBR® Gold Stain 1.1 Dilute the stock SYBR® Gold stain 10,000-fold to make a 1X staining solution. • Dilute into TE (10 mM Tris-HCl, 1 mM EDTA, pH 7.5–8.0), TBE (89 mM Tris base, 89 mM boric acid, 1 mM EDTA, pH 8.0), or TAE (40 mM Tris...
SYBR®Gold Stain 1.1 Dilute the stock SYBR®Gold stain 10,000-fold to make a 1X staining solution. • • Dilute into TE(10 mM Tris-HCl, 1 mM EDTA,pH 7.5–8.0),TBE(89 mM Tris base,89 mM boric acid, 1 mM EDTA,pH 8.0),or TAE(40 mM Tris-acetate, 1 mM EDTA,pH 7.5–8....