Experimental Protocol The protocol below describes how to stain minigels with SYBR® Gold stain after electropho- resis. To stain agarose gels or polyacrylamide minigels, immerse the entire gel in staining solution. To stain large or extremely fragile polyacrylamide gels, leaving the gel on ...
标签或染料SYBR Gold Unit SizeEach 内容与储存 • 以 DMSO 配制的 10,000X 浓缩液形式提供 在-20°C 的干燥器内避光储存。 图表 Comparison of glyoxalated RNA stained with ethidium bromide and with the SYBR® Gold Nucleic Acid Gel Stain. ...
SYBR™ Gold stain has also been shown to be much more sensitive than SYBR™ Green II stain for detecting single strand conformation polymorphism (SSCP) products (see figure below). Furthermore, it is much more sensitive than silver-staining for the detection of double-stranded DNA in native...
As with all nucleic acid reagents, solutions of SYBR®Gold stain should be disposed of in accordance with local regulations. Experimental Protocol resis.To stain agarose gels or polyacrylamide minigels, immerse the entire gel in staining solution.To stain large or extremely fragile polyacrylamide ...
SYBR Safe DNA 凝胶染色剂专门开发用于降低致突变性–,比用溴化乙锭在琼脂糖或丙烯酰胺凝胶中对 DNA 进行染色更安全。SYBR Safe 染料不仅比溴化乙锭具有更低的致突变性,而且 SYBR了解更多信息 Have Questions? 联系我们 更改视图 货号数量 S33100 1 L S33101 4 L ...
The use of SYBR Gold for staining polyacrylamide gels was not satisfactory in terms of the image quality produced. However, it was comparable to silver staining in terms of sensitivity, and could possibly be used in cases where the products are present as sharp single bands. In those cases, ...
The full excitation and emission spectra for SYBR Safe DNA Gel Stain are provided online and can also be found in the protocol provided with the stain. Why do I sometimes see “speckles” in my gel when using SYBR Safe DNA Gel Stain? Many whitening agents used in clothing, as well as...
different melting temperature associated with it, running a melt curve analysis at the end of the PCR is advisable to check the specificity of the reaction. SYBR Green alternatives, like SYBR Gold, SYTO-9, SYTO-82, EvaGreen, etc., are less inhibitory to PCR and can be used to generate ...
The sensitivity, specificity, accuracy, and linearity of the FACS were calculated and compared against Giemsa stained microscopic examination as the gold standard test for detecting malaria parasites. Pearson’s correlation test was used to compare two tests. 3. Results 3.1. Staining of P. falciparum...
In those cases, the use of SYBR(R) Gold gel stain would save time and money for staining polyacrylamide gels.RahmanMuhammad H.JaquishBarryKhasaP.D.Plant Molecular Biology ReporterRahman, M. H., B. Jaquish, and P. D. Khasa. 2000 . Optimization of PCR protocol in microsatellite analysis ...