Figure 1.Principle of detection using Sybr Green. View chapterExplore book Real-time Polymerase Chain Reaction JanetBarletta, inMolecular Aspects of Medicine, 2006 1. SYBR Green I(SGI) is a double-strand (ds) specific, asymmetriccyanine,DNAdye which emits light upon excitation. In solution, the...
A simple fluorescence method to quantitatively monitor malachite green (MG) was introduced based on the conformation switch effect of MG-aptamer (Apt) and the fluorochrome of SYBR Green I (SGI). SGI can embed in the stemloop structure of Apt to produce fluorescence. However, the fluorescence ...
It appears that SGI does not compromise cells for up to 2 h following initial exposure. 展开 关键词: SYBR Green I L23 immune cells Living cells Uptake Fluorescence microscopy Acridine Orange DOI: 10.1016/j.acthis.2005.06.010 被引量: 8 ...
A facile and universal aptamer-based label-free approach for selective and sensitive fluorescence detection of proteins and small biomolecules by using the \\{SYBR\\} Green I (SGI) dye is developed. This robust versatile biosensing strategy relies on fluorescence turn-off changes of SGI, resulting...