We offer an extensive line of SYBR dye products to meet your needs for both real-time PCR and nucleic acid staining applications. Real-time polymerase chain reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data are therefore collected...
We offer an extensive line of SYBR dye products to meet your needs for both real-time PCR and nucleic acid staining applications. Real-time polymerase chain reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data are therefore collected...
Staining Minigels with SYBR® Gold Stain 1.1 Dilute the stock SYBR® Gold stain 10,000-fold to make a 1X staining solution. • Dilute into TE (10 mM Tris-HCl, 1 mM EDTA, pH 7.5–8.0), TBE (89 mM Tris base, 89 mM boric acid, 1 mM EDTA, pH 8.0), or TAE (40 mM Tris...
SYBR Gold 使用说明 下载积分: 100 内容提示: Useful Tips SYBR® Green Nucleic Acid Gel Stains TD 004Revised: 26June2003Useful TipsSYBR Nucleic Acid Gel StainsIntroductionSince their introduction in late 1993, our SYBR ® nucleic acidgel stains have rapidly become very popular due ...
TheCharacterizationofSYBRGoldnucleicaciddye JINLei.(ExperimentalcenterforMedicalBiochemistryand MolecularBiologyTeaching.XuzhouMedicalCollege,Xuzhou,Jiangsu221002,China) Abstract:Objective ItisanalysetwomethodforstainingDNAfragmentsinagarosewithSYBR—Gold.Methods It usedpoststainingandprestainingtOdyedoublestrandDNAinagaros...
方法采用前染色、后染色的方法使用 SYBR-Gold对双链DNA进行染色检测,EB染色作为参照,琼脂糖凝胶电泳为检测手段,凝胶成像系统拍照观察。结果 SYBR-Gold其前染色与后染色对于双链 DNA检测能力均强于 EB染色,但是 SYBR-Gold预混凝胶前染色其分辨效果及DNA迁移速度受到DNA浓度的影响;SYBR-Gold预混样品的前染色方式对于...
We found that SYBR Gold stain is as sensitive as silver staining for detecting DNA-with a single-step staining procedure. Finally, we found that staining nucleic acids with SYBR Gold stain does not interfere with subsequent molecular biology protocols....
SYBR Gold staining is used for enumerating bacteria and viruses in aquatic samples. However, its suitability for epifluorescence microscopy has not been sufficiently investigated. Thus we compared bacterial and viral counts using SYBR Gold and SYBR Green I stains. Variables for both bacterial and vira...
Objective It is analyse two method for staining DNA fragments in agarosewith SYBR-Gold.Methods It used poststaining and prestaining to dye double strand DNA in agarose gel electrophoresis.The same way is used to stain DNA with EB as a control.And we analysed the results by the gel imaging ...
Staining with SYBR®Gold stain is somewhat pH sensitive.For optimal sensitivity,verify that the pH of the staining solution at the temperature used for staining is between 7.0 and 8.5. 1.2 Incubate the gel in 1X staining solution for 10–40 minutes. ...