Also, since the early genic region is normal ( Simian Virus 40 for structure), it may produce the T antigen within the cell. If such a cell is transformed by another SV40 vector in what the viral early gene region was replaced by a foreign piece of DNA, the COS cell may act as a ...
Comparison of the structure of these circular DNAs with those of the integrated p205-GTI copy indicated that large T antigen expression in human cells leads to the amplification of the integrated shuttle vector according to the 'onion skin' model developed for transformed rodent cells. Indeed, ...
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We constructed the eukaryotic specific expression vector of SV40 large T antigen in gastric parietal cells to establish transgenic mouse models with microinjection technique.Methods3.8kbDNA fragment H+-K+ATPase β promoter/SV40T was released from specific expression vector of SV40 large T antigen in ...
Despite the absence of the SV40 T antigen, we found that T antigen knockout clones retained the high-titer phenotype of HEK293T cells for lentiviral vector production. When producing adeno-associated virus (AAV)2 vectors, the HEK293T knockout clones performed similarly to their parental cell cl...
To overcome this challenge, we investigate if piggyBac transposon-mediated expression of SV40 T antigen can effectively immortalize mouse MEFs and that the immortalized MEFs can maintain long-term cell proliferation without compromising their multipotency. Using the piggyBac vector MPH86 which expresses ...
In a BSC-1 cell line containing E-SV40 DNA, subsequent introduction of a vector with a functional viral late coding region (L-SV40) resulted in the appearance of T-Ag and transformation. These findings suggest that L-SV40 sequences activate or enhance T-Ag expression and that this ...
Ef1a_Large T-antigen_Ires_Puro(Plasmid #18922) 慢病毒SV40T表达载体 BACKBONE Vector backbone EGIP Backbone size w/o insert (bp) 10000 Vector type Mammalian Expression, Lentiviral, Cre/Lox Selectable markers Puromycin GROWTH IN BACTERIA Bacterial Resistance(s) ...
1. Production properties and specificity of rabbit antibody to purified simian virus 40 large-T antigen Large quantities of a species of T antigen with an apparent molecular weight of 84,000 have been isolated from monkey kidney cells infected with SV40 by using the protein A Antibody Adsorbent ...
摘要 目的 构建含SV 40LT抗原基因的逆转录病毒载体并转染鼠肝细胞 ,检测SV 40LT抗原基因在肝细胞中的表达情况 ,为肝细胞移植研究打下基础。方法 利用体外基因重组技术构建含SV 40LT抗原基因的逆转录病毒载体并酶切...展开更多 Objective To construct the retroviral vector inserted SV40 large T antigen gene ...