Summary This chapter contains sections titled: Introduction Immortalization SV40 Large T Antigen The Retinoblastoma Family of Proteins The P53 Protein The J Domain and H sc 70 p300 and the CBP Family of Proteins p63 and p73 Family of Proteins Conclusions ReferencesRowena L. Lock...
Immortalization Method:Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen Applications:For Research Use Only Unit quantity:1x10^6cells/ 1.0 ml Cell Type:Immortalized Cells Propagation Requirements:Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular M...
Immortalization Method :Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen Applications :For Research Use Only Unit quantity:1x1066 cells / 1.0 ml Cell Type :Immortalized Cells Propagation Requirements:Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracel...
目的建立猿肾病毒40大T抗原(SV40Tag)永生化尿源性干细胞,为组织工程学及再生医学提供稳定的细胞来源。 方法利用脂质体(Lipofectamine2000)介导将含SV40Tag基因质粒pMPH-FRT-SV40T及转座酶质粒(transposase)共转染至临床分离的尿源性干细胞中。潮霉素(Hygromycin)筛选后阳性细胞克隆并连续传代,观察细胞形态学以及增殖情况...
Immortalization Method Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen Description The Immortalized Lens Epithelial Cells - SV40 is established from primary cells (isolated from infant lens epithelial fragments) using adenovirus-SV40 virus. The Immortalized Human Len...
## SV40 Large T Antigen: Structure and Function. Simian virus 40 (SV40) large T antigen (TAg) is a multifunctional protein that plays a critical role in viral replication, regulation of host gene expression, and immortalization of primary cells. The structure of TAg has been extensively studied...
Transformation of SV40-immortalized human uroepithelial cells by 3-methylcholanthrene increases IFN- and Large T Antigen-induced transcripts 来自 NCBI 喜欢 0 阅读量: 51 作者:LM Crosby,TM Moore,M George,LW Yoon,AB Deangelo 摘要: Background Simian Virus 40 (SV40) immortalization followed by ...
Shay, J.W., Der Haegen, B.A., Ying, Y., Wright, W.E. (1993) The frequency of immortalization of human fibroblasts and mammary epithelial cells transfected with sv40 large t-antigen. Exp. Cell Res. 209: pp. 45-52Shay JW , Van der Haegen BA , Ying Y , Wright WE ( 1993 ) ...
7 Miyazaki M, Mihara K, Bai L et al. Immortalization of epithelial-like cells from human liver tissue with SV40 T-antigen gene. Exp Cell Res, 1993;206(1):27-35 8 吴根生, 陈秋燕, 孙宗棠 et al. SV40 T抗原介导的人胚肝细胞的体外永生化及其机制探讨. 中华肿瘤杂志, 1993;15(6):415-418...
后来人们观察到一些肿瘤细胞具有持续生长和增殖的能力,可长期传代,即细胞的永生化(immortalization)。进一步研究发现部分永生化的肿瘤细胞中含有病毒基因(virusgene),因此通过转染技术将病毒基因导入并整合在体外培养的正常细胞基因组上,使其出现永生化就成为了细胞实验研究的热门。猿猴病毒40(siIllianvims40,SV40)是60...