The immortal state is dependent on the continued presence of T antigen, although whether all of the functions initially required to induce immortalization are required to maintain it is currently unclear. Here,
Summary This chapter contains sections titled: Introduction Immortalization SV40 Large T Antigen The Retinoblastoma Family of Proteins The P53 Protein The J Domain and H sc 70 p300 and the CBP Family of Proteins p63 and p73 Family of Proteins Conclusions ReferencesRowena L. Lock...
SV40 T抗原诱导NLRP3蛋白核转位在角膜上皮细胞永生化中的作用机制研究 《Experimental Eye Research》:NLRP3 proteins translocation into nuclei mediates SV40 T-antigen-induced corneal epithelial cell immortalization 论文解读 在生命科学领域,NLRP3蛋白长期以来被视为细胞质中炎症小体(inflammasome)的核心组分,其经典...
Immortalization Method:Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen Applications:For Research Use Only Unit quantity:1x10^6 cells / 1.0 ml Cell Type:Immortalized Cells Propagation Requirements:Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular...
SV40-Mediated Immortalization Mary Judith Tevethia, Harvey L. Ozer Pages 185-199 Biology ofp53and SV40 Large T Association Ximena Montano Pages 201-211 Binding of SV40 Large T Antigen to the Retinoblastoma Susceptibility Gene Product and Related Proteins ...
Cells that have permanently bypassed the natural senescence point are said to have been immortalized. Immortalization can be brought about by the accumulation of mutations in cellular genes (spontaneous immortalization) or by the introduction of specific types of oncogenes, including T antigen (T-ag)...
Immortalization Method :Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen Cell Type:Immortalized Cells Growth Properties :Adherent Propagation Requirements:Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion...
和增殖的能力,可长期传代,即细胞的永生化(immortalization)。进一步研究发现部分 永生化的肿瘤细胞中含有病毒基因(virusgene),因此通过转染技术将病毒基因导入并整 合在体外培养的正常细胞基因组上,使其出现永生化就成为了细胞实验研究的热门。 猿猴病毒40(siIllianvims40,SV40)是60年代初发现和分离的猴肾细胞病毒,人...
It was known that T antigen expression was sufficient to transform cells by several assays including immortalization, dense focus formation, anchorage independent growth and tumorigenicity in animals. Two models were considered to explain how this single viral protein altered so many cellular properties....
Immortalization Method Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen Description The Immortalized Lens Epithelial Cells - SV40 is established from primary cells (isolated from infant lens epithelial fragments) using adenovirus-SV40 virus. The Immortalized Human Len...