simian virus(SV40 T antigen)immortalizationinsight into wild‐type function of pathwaysSummary This chapter contains sections titled: Introduction Immortalization SV40 Large T Antigen The Retinoblastoma Family of Proteins The P53 Protein The J Domain and H sc 70 p300 and the CBP Family of Proteins ...
The E1A promoter directs the low-level expression of the T antigen in all three germ-layer derivatives7. The E1A-T construct has been shown to promote immortalization but not transformation. It has been successfully used to immortalize cells at the stem cell stage while still allowing ...
Primary PDECs were immortalized with SV40 large T antigen, using ready-to-use Lenti-SV40T (Puro) Lentivirus, High Titer cell immortalization kit (Applied Biological Materials, cat. no. LV613; www.abmgood.com), per the manufacturer’s instructions. The source of the porcine KRASG12D mutant ...
SV40 17KT antigen Transformation Human fibroblasts dnaJ Introduction Simian virus 40 (SV40) was isolated in 1960 from a monkey kidney cell line (Sweet and Hillmon, 1960). Shortly after isolation of the virus, it was shown to cause tumors in rodents (Eddy et al., 1961), leading to the int...
Immortal⁃ ization of neuronal progenitors using SV40 large T antigen and dif⁃ ferentiation towards dopaminergic neurons[ J] . J Cell Mol Med, 2012, 16(11) : 2592-2610.A. Alwin Prem Anand,S. Gowri Sankar,V. Kokila Vani.  Immortalization of neuronal progenitors using SV 40 large ...
Smalley M, Leiper K, Tootle R, McCloskey P, O´Hare MJ, Hodgson H (2001) Immortalization of Human Hepatocytes by Temperature-sensitive SV40 large-T antigen. In Vitro Cell Dev Biol-Animal 37: 166- 168.Smalley M.; Leiper K.; Tootle R.; McCloskey P.; O’Hare M. J.; Hodgson H...
Van der Haegen BA, Shay JW: Immortalization of hu- man mammary epithelial cells by SV40 large T-antigen involves a two step mechanism. In Vitro Cell Dev Biol 1993, 29A:180-182Van der Haegen BA, Shay JW: Immortalization of human mammary epithelial cells by SV40 large T-antigen involves a...
Vitro Cell Dev BiolHaegen BA, Shay JW: Immortalization of human mammary epithelial cells by SV40 large T-antigen involves a two step mechanism. In Vitro Cell Dev Biol 1993, 29A (3 Pt 1) : 180–182.Van Der Hagen, B. A. and J. W. Shay. Immortalization of human mammary epithelial ...
All cell lines tested retained the ability to increase AKT and SMAD2 phosphorylation in response TGF-β1 treatment. Conclusions: These results indicate that fibroblast immortalization through a combined SV40 large T antigen and hTERT expression approach is superior to individual manipulation. The ...
SV40 T antigen induces karyotype instability soon after it is expressed in human diploid fibroblasts and ultimately promotes cell immortalization and tumorigenesis. Protein levels and activities of mitotic cell cycle proteins have been shown to be elevated in several immortal cell lines relative to...