Invitrogen SuperScript IV 一步法 RT-PCR 系统兼具 SuperScript IV 反转录酶的高持续合成能力和 Platinum SuperFi DNA 聚合酶的高保真度,可提供无可比拟的一步法 RT-PCR 性能。使用该便利的一步法配方加基因特异性引物,您可在单管中从总 RNA 或 mRNA 中进行 cDNA 合成和 PCR 扩增。
The Invitrogen SuperScript One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
一步法RT-PCR和两步法RT-PCR是两种最常用方法。顾名思义,一步法RT-PCR在单个反应管中将第一链cDNA合成和后续PCR反应结合在一起。相比两步法RT-PCR,一步法RT-PCR实验流程更简单快速,污染风险更低,也更适合高通量实验应用。 当季上市新品 Invitrogen™ SuperScript™ IV UniPrime™ 一步法RT-PCR系统提供方便...
1. Program the thermal cycler so that cDNA synthesis is followed immediately with PCR amplification automatically.Note: The following cycling conditions were established using a DNA Thermal Cycler 9600 or 2400 (Perkin-Elmer) and may have to be alteredfor other thermal cyclers. Efficient cDNA ...
SuperScript IV 一步法 RT-PCR 系统结合了 SuperScript IV 反转录酶的高持续合成能力和 Platinum SuperFi DNA 聚合酶的高保真度,以便在更短时间内和更宽靶标范围内提供无与伦比的产物产量、特异性和灵敏度,即使使用纯度不佳的 RNA 样品也是如此。 仅供科研使用。不可用于诊断程序。 规格 检测方法引物-探针 产品...
The SuperScript™ III One-Step RT-PCR System with Platinum® Taq High Fidelity is designed for sensitive, high-fidelity end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a sing...
品名: SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase 规格: 100 reactions 内容及储存 See Table 1 for components. Store at -20°C. Guaranteed stable for 6 months when properly stored. 产品描述 The SuperScript III One-Step RT-PCR System with Platinum Taq DNA ...
2 SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase Product Information Sheet Troubleshooting Observation No amplificiation product Low specificity Unexpected bands after electrophoretic analysis Possible cause No cDNA synthesis (temperature too high) RNase contamination Not ...
SuperScript IV One-Step RT-PCR System Other provider one-step RT-PCR kit I am currently not using one-step RT-PCR kits 3. What applications do you perform after one-step RT-PCR? * DNA cloning DNA electrophoresis DNA sequencing NGS
Invitrogen SuperScript IV UniPrime 一步法 RT-PCR 系统是一种便捷的一步法配方,可在单管中进行快速 cDNA 合成和 PCR 扩增。 Have Questions? 更改视图 货号描述反应次数 12596100无染料100 × 50 μL 反应 12596025无染料25 × 50 μL 反应 12597025显色25 × 50 μL 反应 ...