SUMOylated proteins that are targeted for degradation by RNF4 should be enriched upon RNF4-knockdown and be a direct ubiquitylation target for RNF4-TULIP, enriched after proteasome inhibition in a SIM-dependent
The SPRING domain, which resembles the RING domain found in ubiquitination E3 ligases, binds to Ubc9 and is essential for SUMO E3 ligase activity. In addition, SP-RING proteins contain a SAP [Scaffold Attachment factor (SAF)-A/B, acinus, PIAS] motif (DNA binding), a PINIT motif (pro...
These findings suggest that the centromere complex is under continued surveillance by SUMO E3 ligases that control the localization of centromere proteins which is counteracted selectively by SENP6 (Supplementary Fig. 5). Using SUMO pull-down experiments from cells in which we can control SENP6 ...
SUMOylation of target substrates requires specific conjugation machinery to occurin vivo, and E3-SUMO ligases facilitate substrate specificity and enhance SUMO transfer from the E2-SUMO-conjugating enzyme, Ubc9. We tested whether GβL could be SUMOylated using purified recombinant E1 and E2in vitro...
(SUMO E3 ligase)的依次作用下共价结合到底物蛋白 的赖氨酸(K, Lys)上, 完成SUMO化修饰.由于SUMO 结合酶E2可直接与底物蛋白互作, 体外SUMO化反应 通常不需要添加E3连接酶, 但SUMO连接酶E3在体 内可提高SUMO化修饰的效率和特异性(Bernier-Villa- mor et al., 2002).SUMO化修饰过程可逆, 在SUMO 蛋白酶的...
E3 ligases, such as PIAS and RanBP2 [12]. In a previous study, Ubc9 was shown to interact with HIV-1 IN, and SUMO2/3 and Ubc9 negatively regulate the HIV-1 integration step [13]. Similar to ubiquitination, the substrate proteins can be poly-SUMOylated or mono-SUMOylated at single ...
The mature form of SUMO is conjugated to the target proteins by a three-step enzymatic cascade, very similar to the ubiquitin pathway but involving different enzymes: E1 activating enzyme, E2 conjugating enzyme and E3 ligases (Figure 1). ...
catalyzedbySUMO-specificE1,E2,andE3ligasesandcanbereversedbySUMO-specific proteases(SENPs).Inmammaliancells,sixSENPshavebeenidentified.Theycanbe dividedintothreesubfamiliesonthebasisoftheirsequencehomology,cellularlocalization andsubstratespecificity(Yeh,2009).SENP1belongstothefirstsubfamily,which ...
(SUMO E3 ligase)的依次作用下共价结合到底物蛋白 的赖氨酸(K, Lys)上, 完成SUMO化修饰.由于SUMO 结合酶E2可直接与底物蛋白互作, 体外SUMO化反应 通常不需要添加E3连接酶, 但SUMO连接酶E3在体 内可提高SUMO化修饰的效率和特异性(Bernier-Villa- mor et al., 2002).SUMO化修饰过程可逆, 在SUMO 蛋白酶的...
The conjugation of SUMO to target proteins requires an E1-activating enzyme (SAE1/2), an E2-conjugating enzyme (UBC9), and one of several E3 SUMO ligases4. Unlike ubiquitination, in vitro SUMOylation can occur without E3 SUMO ligases, although enhanced substrate specificity is conferred by ...