The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Sulforhodamine B, an anionic aminoxanthene dye, can form an electrostatic complex with the basic amino acid residues of protei...
应用于荧光染色技术。磺酰罗丹明B已被用作MCF-7(人乳腺癌细胞系)细胞增殖实验的染料 Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoich...
Sulforhodamine B (SRB) cell cytotoxicity assay has been widely used for drug toxicity screening and cell proliferation detection against different types of cancerous and non-cancerous cell lines. In addition, this assay does not depend on the metabolic activity of the cells and therefore shows less...
英文名称Sulforhodamine B, acid form 产品介绍 磺酰罗丹明 B (SRB) 是一种荧光蛋白染料。 据报告,染料在温和的碱性条件下与三氯乙酸固定细胞的氨基酸残基结合,并在温和的酸性条件下将其自身提取到溶液中。 最大吸收波长: 558 nm 产品应用: SRB 对其对活细胞反应性的 pH 敏感性使其可用于药物诱导的细胞毒性和...
2.4 Sulforhodamine B assay Sulforhodamine B (Fig. 10.4) is a fluorescent dye with uses spanning from laser-induced fluorescence to the quantification of cellular proteins of cultured cells. The SRB assay is based on binding of the dye to basic amino acids of cellular proteins, and colorimetric ...
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Its principle is based on the ability of the protein dye sulforhodamine B to bind electrostatically and pH dependent on ...
Sulforhodamine B Sulphorhodamine B (SRB) assay 1. Seed cells in a volume of 100ul/well in 96-well plate. Pre-incubate overnight.2. Treat cells according to different experimental purposes*.3. Pour off the medium and fix cells by adding 100ul of cold 10% TCA. Incubate for 60 min or ...
[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. 公司简介 武汉科斯坦生物科技有限公司是一家致力于前沿生物技术研发与成果转化的创新型高科技企业。
SRB法 1. Correlation between experimental results of MTT colorimetric assay and SRB assay in anti-tumor drug screening in vitro SRB法和MTT法抗肿瘤药物筛选结果相关性研究 2. Methods The drug-sensitivity in 37 cases of primary culture of oral maxillofacial squamous cell carcinoma from the patients ...
SRB assay was used to monitor the enzymatic activity of living cells. General Description Sulforhodamine B (SRB) is a fluorescent protein dye. It was reported that the dye binds itself to the amino acid residues of tricholoroacetic acid fixed cells in mild basic conditions and extracts itself ...