The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-screening applications. Sulforhodamine B, an anionic aminoxanthene dye, can form an electrostatic complex with the basic amino acid residues of protei...
Sulforhodamine B (SRB) cell cytotoxicity assay has been widely used for drug toxicity screening and cell proliferation detection against different types of cancerous and non-cancerous cell lines. In addition, this assay does not depend on the metabolic activity of the cells and therefore shows less...
A simple test system to monitor the effects of test compounds on proliferation of cultured cancer cells is the sulforhodamine B (SRB) assay, developed by Skehan et al. (1990). The SRB assay is based on the ability of the protein dye SRB to bind electrostatically and pH-dependent to protein...
[1]. Orellana EA, et al. Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation. Bio Protoc. 2016 Nov 5;6(21). pii: e1984. 公司简介 武汉科斯坦生物科技有限公司是一家致力于前沿生物技术研发与成果转化的创新型高科技企业。
To overcome restrictions on the use of fish in toxicity testing, the present study proposes to compare the 50% growth inhibition potential (EC50) of four types of effluents on the rat cardiomyoblast H9c2(2-1) cell line by using the sulforhodamine B (SRB) cell mass colorimetric assay, with ...
Their effects on growth inhibition against HL-60,HeLa and SGC-7901 cells were determined by SRB assay. 用SRB法测定目标化合物对癌细胞的增殖抑制作用。 更多例句>> 3) SRB method SRB法 1. Their growth inhibitory activity to tumor cells were detected by SRB method,and a positive reaction was...
However, within a given oxygen environment, both proliferation assays show a correlation with cell number. Therefore, the MTT assay must be used with caution when comparing cell growth or drug response for cells grown in different oxygen environments. 展开 ...
Various assays are used to evaluate the cytotoxic effect of chemicals on cultured cells. The sulforhodamine B (SRB) colorimetric assay is based on the ability of the SRB dye to bind basic amino acid residues on proteins. In contrast, the MTT (dimethylthi
Colourimetric methods in general, and the sulforhodamine B (SRB) assay in particular [36], appears to be the most convenient alternative to hemacytometer counting. The SRB binds to the basic amino acids of cellular macromolecules. The SRB assay is commonly used for cell determination in monolayer...
The inhibition of the EGFR activity by the compound synthesized was evaluated and analyzed by the sulforhodamine B (SRB, Sigma) assay for their inhibitory activities toward human epidermoid carcinoma (A-431) and breast cancer (MDA-MB-468) cells. These cells are known to overexpress EGFR, which...