Sucrose 6-phosphate hydrolase is found in S . mutans cells cultured on a number of sugars; however, the best yield is obtained from sucrose-grown cells. Sucrose 6-phosphate and sucrose are the only known substrates of the enzyme. Among the buffers tested, 2-( N -Morpholino)ethanesulfonic ...
This chapter describes the assay method, the purification procedure, and properties of sucrose-6-phosphate hydrolase from Streptococcus mutans . The continuous spectrophotometric assay is based upon the observation that when glucose-6-phosphate dehydrogenase is present in excess, the rate of sucrose 6-...
Phospho-α-glucosidase and sucrose-6-phosphate hydrolase exhibit no significant homology, and by sequence-based alignment, the two enzymes are assigned to Families 4 and 32, respectively, of the glycosyl hydrolase superfamily. The phospho-α-glucosidase gene (aglB) lies adjacent to a second gene ...
Rauch PJG, de Vos WM (1992) Transcriptional regulation at the Tn5276-locatedLactococcus lactissucrose operon and characterization of thesacAgene encoding sucrose-6-phosphate hydrolase. Gene 121:55–61 PubMedGoogle Scholar Ruijter GJG, Van Meurs G, Verwey MA, Postma PW, van Dam K (1992) Analysi...
Moreover, phylogenetic and sequence analysis suggest that glycoside hydrolase 13 subfamily 18 might harbour even more specificities. In addition, the second gene residing in the same operon as sucrose 6′-phosphate phosphorylase was identified as well, and found to be a phosphofructokinase. The ...