Sucrose 6-phosphate hydrolase is found in S . mutans cells cultured on a number of sugars; however, the best yield is obtained from sucrose-grown cells. Sucrose 6-phosphate and sucrose are the only known substrates of the enzyme. Among the buffers tested, 2-( N -Morpholino)ethanesulfonic ...
2001. Metabolism of sucrose and its five linkage- isomeric a-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-a-glucosidase. J Biol Chem. 276:37415-37425.John T,Stanley A,Frieder W,et al.Phosphorylation and metabolism of ...
The sucrose-6-phosphate hydrolase (scrB), which was from a screened strainThermoanaerobacterium thermosaccharolyticumG3-1 was overexpressed inT. aotearoenseSCUT27 and endowed this strain with the ability to utilize sucrose. In addition, overexpression of the sucrose-specific PTS system (scrA) fromC...
Intracellularly, sucrose-6-phosphate (sucrose-6-P) is hydrolyzed by sucrose-6-phosphate hydrolase (8, 9) to glucose-6-phosphate and fructose, which are then fermented via the glycolytic pathway to yield primarily lactic acid. The structures of sucrose, its five isomeric α-d-glucosyl-d-...
6.to form sugar or sugar crystals. 7.to make maple sugar. 8.sugar off,(in making maple sugar) to complete the boiling down of the syrup in preparation for granulation. [1250–1300; Middle Englishsugre, sucre(n.) < Middle Frenchsucre< Medieval Latinsuccārum< Italianzucchero< Arabicsukkar...
A recombinant vector comprises a ptsG gene and sacC gene which codes sucrose-6-phosphate hydrolase. The sacC gene is denoted by the sequence number 4. A recombinant microorganism having sucrose metabolic activity is produced by transforming bacteria, yeast or fungus with the recombinant vector. 展开...
Invertase, a hydrolase, including acid invertase (AI) and neutral invertase (NI), can cleave sucrose into glucose and fructose, and SS, a glycosyl transferase, con- verts sucrose into UDP-glucose and fructose in the presence of UDP12. In source tissues, sucrose phosphate syn- thase (SPS) ...
6 type. Purified Phosphorylase synthesizes amylose chains alone from α-glucose-1-phosphate. In the degradation of glycogen, Phosphorylase will degrade amylose chains to the branch points. An amylo-1, 6-glucosidase is then required to cleave the 1, 6 bonds; such cleavage then provides an entry...
Sucrose phosphate synthase is assayed by the measurement of fructose-6-phosphate-dependent formation of sucrose from UDP-glucose (Huber and Bickoff, 1984). From: Phenotyping Crop Plants for Physiological and Biochemical Traits, 2016 About this pageAdd to MendeleySet alert ...
The majority of sucrose-positive bacteria take up and phosphorylate sucrose by a phosphoenol pyruvate-dependent, sucrose-6-phosphotransferase system (sucrose PTS) to yield intracellular sucrose-6-phosphate. This phosphate is hydrolyzed by a sucrose-6-phosphate hydrolase (invertase or sucrase) into D-...