substrate-assisted catalysisG-proteinsRasserine proteasesrestriction endonucleasesIn enzyme catalysis, the convention is that the enzyme supplies all the functional groups that are needed to convert a substrate into a product. This convention, however, is now recognized to have some exceptions. In a ...
The idea that both the substrate and the enzyme contribute to catalysis (substrate assisted catalysis; SAC) is applicable to guanine nucleotide-binding proteins (G proteins). Naturally occurring SAC uses GTP as a general base in the GTPase reaction catalyzed by G proteins. Engineered SAC has ident...
Quantum Mechanics/Molecular Mechanics Modeling of Substrate-Assisted Catalysis in Family 18 Chitinases: Conformational Changes and the Role of Asp142 in Catalysis in ChiB 来自 Semantic Scholar 喜欢 0 阅读量: 64 作者: Jitrayut Jitonnom, Vannajan S. Lee, Piyarat Nimmanpipug, Heather A. ...
Histidine 57 of the catalytic triad of was replaced with alanine to determine whether the resulting variant would be capable of substrate-assisted catalysis [Carter, P., & Wells, J. A. (1987) Science 237, 394-9]. A 2.5-fold increase in kcat/Km was observed on tri- or substrates contain...
Substrate-assisted catalysis: molecular basis and biological significance. SAC has been demonstrated for representatives of three major enzyme classes: serine proteases, GTPases, and type II restriction endonucleases, as well as ... W Dall'Acqua,P Carter - 《Protein Science》 被引量: 91发表: 201...
Computer-aided design of a catalyst for Edman degradation utilizing substrate-assisted catalysis 来自 掌桥科研 喜欢 0 阅读量: 54 作者:B Borgo,JJ Havranek 摘要: Molecular biology has been revolutionized by the miniaturization and parallelization of DNA sequencing assays previously performed on bulk ...
Structural and Biochemical Evidence That a TEM-1 OE≤-Lactamase N170G Active Site Mutant Acts via Substrate-assisted Catalysis. J Biol Chem. 2009;284(48):33703-12.Brown NG, Shanker S, Prasad BV, Palzkill T. Structural and biochemical evidence that a TEM-1 beta-lactamase N170G active ...
Williams SJ, Mark BL, Vocadlo DJ, James MN, Withers SG: Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. J Biol Chem. 2002, 277: 40055-40065. 10.1074/jbc.M20...
Substrate-Assisted Catalysis substrate-enzyme complex Substrate-level phosphorylation Substrate-level phosphorylation Substrate-level phosphorylation Substrate-Removed Substrate-Triggered Silicon-Controlled Rectifier substrates substrates substrates substrative substrative ...
Instead, they use substrate-assisted catalysis to transfer a single ADP-ribose unit onto substrates [3]. During this process the activating glutamate of the substrate is subsequently ADP-ribosylated, consequently the modified glutamate is not available for a following second catalytic step and thus ...