DNADNA Directed RNA Polymerase metabolismEscherichia coliNucleic Acid Conformation大肠杆菌核酸构象转录, 遗传It was previously thought that elongating Escherichia coli transcription ternary complex consists of an RNA polymerase molecule enclosing 17 +/- 1 melted bases (bubble) of the template DNA and a ...
Distinguish between the three types of RNA: mRNA, tRNA, and rRNA. How does the primary transcript (pre-mRNA) in the nucleus of a eukaryotic cell compare to the functional mRNA? Compare and contrast DNA and RNA molecules in vivo in terms of their secondary and tertiary structures. ...
The most stable structure of DNA is the canonical right-handed double helix termed B DNA. However, certain environments and sequence motifs favor alternative conformations, termed non-canonical secondary structures. The roles of DNA and RNA secondary structures in transcriptional regulation remain incomple...
Molecular structure of r(GCG)d(TATACGC): a DNA--RNA hybrid helix joined to double helical DNA. The molecule r(GCG)d(TATACGC) is self-complementary and forms two DNA–RNA hybrid segments surrounding a central region of double helical DNA its molecular... HJ Wang,S Fujii,JH Van Boom,....
DNA ligase IV (LigIV) performs the final DNA nick-sealing step of classical nonhomologous end-joining, which is critical for immunoglobulin gene maturation and efficient repair of genotoxic DNA double-strand breaks. Hypomorphic LigIV mutations cause extr
DNA-RNA and DNA-DNA duplexes are even more polymorphic than observed previously. DNA-RNA hybrids can have secondary structures like A-DNA or A-RNA, but double helices of the synthetic DNA-RNA hybrids poly(dA) X poly(rU) and poly(dI) X poly(rC), respectively, form 11-fold and 10-fol...
Biosensors based on DNA or RNA aptamers (aptasensors) are of considerable interest as an alternative to the biosensors based on antibodies. Aptamers are artificial oligonucleotides (DNA or RNA) that at certain conditions (ionic composition, pH, temperature) adopt three dimensional structure with binding...
Moreover, LONP1 possesses ATP-dependent, protease-independent chaperone activity and works in concert with the mtHSP70-DNAJA3 chaperone system to solubilize a significant portion of imported mitochondrial proteins, including OXAL1, NDUFA9, and CLPX11,12,13. Homozygous deletion of Lonp1 in mice ...
RNA molecules are sequences of nucleotides that serve as more than mere intermediaries between DNA and proteins, e.g. as catalytic molecules. Computational prediction of RNA secondary structure is among the few structure prediction problems that can be solved satisfactory in polynomial time. Most work...
Here, we describe a web server built around ARTS, a method for aligning tertiary structures of nucleic acids (both RNA and DNA). ARTS receives a pair of 3D nucleic acid structures and searches for a priori unknown common substructures. The search is truly 3D and irrespective of the order ...