The plasma membrane structure is a lipid bilayer that separates the interior of the cell from the exterior of the cell. Here we describe cell membrane markers that outline the cell boundary for fluorescence imaging. Fluorescent labeled lectins (e.g., wheat germ agglutinin (WGA)) can identify ce...
a cytoskeleton like structure that sits immediately under the plasma membrane in mature merozoites), IFAs were repeated with purifiedP. knowlesimerozoites using both permeabilising and non-permeabiling conditions. To validate the permeabilisation process the staining...
Endoplasmic reticulum (ER)-plasma membrane (PM) junctions are contact sites between the ER and the PM; the distance between the two organelles in the junctions is below 40nm and the membranes are connected by protein tethers. A number of molecular tools and technical approaches have been recently...
Endoplasmic reticulum (ER)-plasma membrane (PM) junctions are contact sites between the ER and the PM; the distance between the two organelles in the junctions is below 40nm and the membranes are connected by protein tethers. A number of molecular tools and technical approaches have...
Cold atmospheric plasma (CAP) has great potential for sterilization in the food industry, by deactivation of thermophilic bacteria, but the underlying mechanisms are largely unknown. Therefore, we investigate here whether CAP is able to denature/modify p
12、ane: Some substance cross the plasma membrane by simple diffusion down their concentration gradient. Others require membrane transport proteins to provide them with individual passage across the plasma membrane. Vesicular Transport Vesicular transport-it is a process that involves configurational ch 13...
Plasma Membrane Structure WGA conjugates, cell membrane stains, and fluorescent fusion proteins for staining cell membranes. Additional ways to label cell structures and cellular components: CellLight Fluorescent Protein Labeling CellLight fusion proteins provide an easy way to label specific structures in...
with very different physical properties are assembled by the cell to make a strong yet extensible wall. This Review describes the physics of wall growth and its regulation by cellular processes such as cellulose production by cellulose synthase, modulation of wall pH by plasma membrane H+-ATPase,...
When budding is at the cell plasma membrane, the glycoproteins are transported via the Golgi apparatus to the cell surface. Some enveloped viruses mature at intracellular membranes, and in these cases the glycoproteins are directed to the appropriate place in the cell. Both Type I integral ...
A plasma membrane surrounds the cytoplasm and lies next to the cell wall. Reproduction Asexual spores are formed by the aerial mycelium of one organism. When these spores germinate, they become organisms that are genetically identical to the parent. Sexual spores result from the fusion of nuclei ...