The primary structure of Providencia rettgeri penicillin G amidase gene and its relationship to other gram negative amidases. DNA Seq - Ljubijankic, Konstantinovic, et al. - 1992 () Citation Context ...lyzing aliphatic amides (IIb). Class IIa enzymes that have been sequenced are those from ...
Cell culture. The human embryonic kidney 293 T cells used in this study were maintained in DMEM supple- mented with 100 U/mL of penicillin, 100 μg/mL of streptomycin and 10% (v/v) FBS, at 37 °C and 5% CO2. DsRed-eGFP surrogate reporter assay. HEK293T cells...
Molecular docking study was analyzed for the compound 1 and 2 with Penicillin binding proteins (PDB:1MWT) and Staphylocoagulase (PDB:1NU7) of MRSA. Compound 1 showed -6.0 kcal/mol for both Penicillin binding proteins and Staphylocoagulase while compound 2 showed the binding score -6.9 kcal/...
HEK-293T-hPOT1-KO WT, EV, and mutant cell lines were passaged and cultured in Dulbecco’s modified Eagle’s Medium (high glucose) supplemented with 10% fetal bovine serum (FBS) (Hyclone), penicillin–streptomycin (100 U/ml, Hyclone), and puromycin (1 μg/ml, InvivoGen). The medium...
The severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne human-infecting bunyavirus, which utilizes two envelope glycoproteins, Gn and Gc, to enter host cells. However, the structure and organization of these glycoproteins on virion
In brief, EGM2 is composed of 20% FBS, 1% penicillin–streptomycin, 1% GlutaMAX (Gibco, 35050061), 1% ECGS (endothelial cell growth factor), 1 mM sodium pyruvate, 7.5 mM HEPES, 0.08 mg ml–1 heparin and 0.01% amphotericin B in a mixture of 1× RPMI-1640 with and ...
The adherent human HEK293T cell line (ATCC, no. CRL-3216) was cultured at 37 °C, 5% CO2in DMEM (Gibco, no. 41965-039) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS), 1 mM sodium pyruvate, 4 mMl-glutamine, 50 U ml−1penicillin, 50 μg...
In the last step of PG synthesis, PG monomer units are polymerized via transpeptidation and transglycosylation reactions, which take place outside the cytoplasmic membrane (Figure 2). The major proteins involved in PG assembly are called penicillin-binding proteins (PBPs) because they are targets...
Overnight cultures grown in LB broth containing 150 mg/L penicillin G (Sigma) were diluted 100-fold into fresh LB-penicillin broth and grown at 37° C. until midlog phase (OD of 0.8 at 600 nm) at which time they were chilled to 24° C. and adjusted to 50 μM IPTG (isopropyl β...
ChemInform Abstract: STRUCTURE‐ACTIVITY RELATIONS IN CEPHALOSPORINS PREPARED FROM PENICILLINS. 1. 7β‐ACYLAMINO DERIVATIVES OF 3‐BENZYL‐ AND 3‐(3‐PYRIDYLMETHYL)CEPH‐3‐EM‐4‐CARBOXYLIC ACIDSdoi:10.1002/chin.197749388BRAIN E. G.EGLINGTON A. J....