Crystal structure of isopenicillin n synthase is the first from a new structural family of enzymes. Nature, 375(6533): 700-704, June 1995.Roach PL, Clifton IJ, Fulop V, Harlos K, Barton GJ, Hajdu J, Anders- son I, Schofield CJ, Baldwin JE: Crystal structure of isopenicillin N ...
HEK293T and U2OS cells (ATCC) were cultured at 37 °C and 7% CO2 in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich, D6546) with GlutaMax (2 mM), penicillin (100 μg/mL, Duchefa), streptomycin (100 μg/mL, Duchefa), and 10% (v/v) newborn calf serum (Seradigm). The med...
The peptide antibiotic ramoplanin factor A2 is a promising clinical candidate for treatment of Gram-positive bacterial infections that are resistant to antibiotics such as glycopeptides, macrolides, and penicillins. Since its discovery in 1984, no clinical or laboratory-generated resistance to this ...
Fisher's exact test was employed to analyze antibiotic choices, which were categorized based on the presence or absence of penicillin allergy in the study population.During the period spanning May 1, 2019, to April 30, 2020, 406 patients with a diagnosis of GBS positivity experienced labor. Of...
HeLa (ECACC 93021013) and HEK293T (ECACC 85120602) cells were grown in DMEM (Sigma D6429), supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; Sigma F9665) and 1% (v/v) penicillin/streptomycin (Sigma P0781; with 10,000 units penicillin and 10 mg streptomycin per ml...
of Peredox, resulting in a family of constructs termed NAPstars (Supplementary Table1). To gain insight into the potential structure of NAPstar, we used AlphaFold2 with the 919 amino acids of NAPstar3 as the input (Supplementary Information). The sequence consists of a Rex domain linked ...
Cells were plated at a density of 5 × 105 on poly-D-lysine-coated (500 μg/ml in RNase free water) dishes and 1 × 105 glass coverslips. Cultures were plated in Neurobasal medium (Invitrogen) supplemented with 10% fetal bovine serum and penicillin/streptomycin mix and grown ...
NEP1-like proteins (NLPs) are a novel family of microbial elicitors of plant necrosis. Some NLPs induce a hypersensitive-like response in dicot plants though the basis for this response remains unclear. In addition, the spatial structure and the role of these highly conserved proteins are not...
Strains lacking CydDC have also been shown to exhibit sensitivity to benzylpenicillin and loss of motility [53], which was hypothesised to be due to defective disulfide folding of penicillin binding protein 4 and the P-ring flagellar protein, respectively. To further characterise the pleiotropic cy...
PC-3 cells (European Collection of Cell Cultures, Salisbury, UK), established from a human prostate adenocarcinoma, were cultured in Coon's modified Ham's F-12 medium (Sigma) containing 10% (v/v) heat-inactivated fetal bovine serum, 100 units/ml penicillin, and 100 µg/ml streptomycin....