RNAs perform their function by forming specific structures, which can change across cellular conditions. Structure probing experiments combined with next generation sequencing technology have enabled transcriptome-wide analysis of RNA secondary structure
weak structural modifications and stabilization may be readily destroyed. As an example, in an initiator transfer RNA (tRNA) molecule that lacks a methyl group (tRNAiMet), modification at position 58 of the tRNA chain renders the molecule unstable and hence nonfunctional; the nonfunctional chain ...
Structure determination is a procedure by which the three-dimensional atomic coordinates of a molecule or biomolecule are solved using an analytical technique. Many techniques are used in structure determination, most commonly X-ray crystallography, NMR spectroscopy, electron microscopy and molecular modelli...
Under an Elsevier user license open archiveAbstract Eukaryotic photosystem I consists of two functional moieties: the photosystem I core, harboring the components for the light-driven charge separation and the subsequent electron transfer, and the peripheral light-harvesting complex (LHCI). While the ...
of an mRNA, where an I is recognized as a G or a C as a U after deamination, it can lead to amino acid substitutions (called ‘recoding’) and enhance the diversity of the proteome. However, it is important to note that RNA editing is primarily carried out in noncoding regions of ...
An unusual RNA molecule encoded by the Bacillus subtilis bacteriophage phi 29 is a structural component of the viral prohead and is required for the ATP-de... S Bailey,J Wichitwechkarn,D Johnson,... - 《Journal of Biological Chemistry》 被引量: 129发表: 1990年 ...
Genome-wide probing of RNA structure reveals active unfolding of mRNA structures in vivo. RNA has a dual role as an informational molecule and a direct effector of biological tasks. The latter function is enabled by RNA's ability to adopt comple... Rouskin,Silvi,Zubradt,... - 《Nature》 ...
When an mRNA molecule carrying the information for a particular protein – that is, a piece of mRNA corresponding to a gene – is complete, it still needs to be processed before it is ready to do its job of delivering a chemical blueprint to the ribosomes, where protein synthesis takes ...
33. The structure of PSII–ACPII was resolved by cryo-EM single-particle analysis, achieving an overall resolution of 2.53 Å (Fig.S2; TableS1). The cryptophyte PSII–ACPII supercomplex exhibited dimeric features, with a dimension of approximately 245 × 190 × 115 ų and a ...
structure of an RNA and its ability to interact with proteins. Analysing in silico, in vitro and in vivo experiments, we find that the amount of double-stranded regions in an RNA correlates with the number of protein contacts. This relationship —which we call structure-driven protein ...