This protocol describes a technique to image different cell populations in draining lymph nodes without alterations in the organ structure. Tags Lymph Node StructureCellular LocalizationEx-vivo Confocal MicroscopyFluorescent Labeled AntibodiesSecondary Lymphoid OrgansProtocolVisualizationExecutionMouse HandlingTissue ...
while B lymphocytes can be isolated from suspensions of spleen or lymph node cells excised from immunized animals, they have a limited life span and cannot be cultured directly to produce antibody in useful amounts. Fortunately, this restriction has b...
A, SEC of recombinant proteins equating to the complete (aa 60–189) or incomplete (aa 93–189) PH domain of TRIOBP-1. The complete domain exists as a single clear species, whereas the incomplete domain exists as multiple species, labeled A–C, including one in the void volume. B, CD...
Although a lot is known about how Fibroblastic Reticular Cells (FRCs) can regulate T lymphocytes (T cells), little is understood about whether or how T cells can regulate FRCs. This study shows that the absence of T cells inhibited the secretion of ER-TR
This region was then subdivided and labeled as separate objects. The global angle of each object is then compared to the global angle of the k-nearest fiber objects, via the same metric described in the fiber-fiber method. Prediction of Recurrence To predict recurrence, we compared tissue ...
A basic protein has been purified from the CNS myelin of the gummy shark (Mustelus antarticus). Electroblotting was used to examine the capacity of rabbit antisera raised against this electrophoretically pure protein to recognize myelin basic protein from higher vertebrates. The antisera bound to ...
The peak specific activity (SA) of HDL apoA-I derived from (131)I-labeled chylomicra occurred at 3 hr after injection (172 +/- 11% of 1 min S... JS Parks,LL Rudel - 《Journal of Lipid Research》 被引量: 176发表: 1982年 Lymphocyte recognition of lymph node high endothelium. III....
The two sub-domains of Set1 are labeled as SET-N/C and SET-I. (C) Alternate view of the Set1-Swd1-Swd3 interface highlighting the crevasse formed between Set1 (blue) and Swd1-WDRP loop (pink), both of which are shown in surface representation. The residues from Swd1 that line ...
Fluorescein and horseradish peroxidase-labeled monoclonal antibodies were used to localize the predominant toxic peptide CSTX-1 in the venom gland of the s... Heinz,Malli,Lucia,... - 《Cell & Tissue Research》 被引量: 51发表: 2000年 Effects of Microwave, Steam and Water Blanching on Freeze...
Provided is a therapeutic method exclusively targeting an amyloid β protein (Aβ) having a specific turn structure of Aβ. Specifically provided is an antibody which specifically recognizes an amyloid β having a turn structure at amino acids positions 22 and 23. Also provided are a medicinal co...