The restriction fragments were blunt-ended and biotinylated with biotin-14-dCTP, diluted, and ligated with T4 ligase at 16°C for 4 h. After the rever- sal of crosslinks at 65°C overnight, the ligated DNA was purified and sheared to lengths of 300–500 bp with Covaris M220, pulled ...
High-order chromatin structure plays important roles in gene regulation. However, the diversity of the three-dimensional (3D) genome across plant accessions are seldom reported. Here, we perform the pan-3D genome analysis using Hi-C sequencing data from
of the MOMP gene. or its fluorescent analogous sequence, can be synthesized enzymatically using dATP or dFTP, respectively, in the presence of DNA polymerase, dCTP, dTTP, and dGTP: MOMP GENE SEQUENCE SEQ ID NO: 1: AAC GTT CGA GAC GGA CAC CCC TTA GGA CGA CTT GOT TCG ...
The formula is below:FSn(Bi,Bj)=CSn(Bi,Bj)/TSn(totalcis+totaltrans)F refers to the relative trans-interaction frequency. Sn refers to stage n. (Bi, Bj) refers to interactions between bin i and bin j. C refers to the normalized interaction count. T refers to the total number of ...