This is accompanied by large-scale reorganization of the inhibitory RFTS and CXXC domains, allowing the enzyme to gain full activity. Our results therefore provide a mechanistic basis for the activation of DNMT1, with consequences for basic research and drug design....
During the revision of this manuscript, the crystal structure of DNMT1–DNA complex was reported21. In this structure, the CXXC domain is also inserted into the major groove of the CpG DNA and causes the major groove widening (Supplementary Fig. S3). In addition, it was reported that the...
1b, Supplementary Fig. 1b). Recent studies showed that the RNA–DNA base pairing at the PAM-distal region (i.e., 20-bp RNA–DNA heteroduplex formation) is important for the activation of the HNH nuclease domain in SpCas921,22, suggesting that CdCas9 requires the 22-bp, rather than ...
ISfinder: the reference centre for bacterial insertion sequences. Nucleic Acids Res. 34, D32–D36 (2006). Article CAS PubMed Google Scholar Barabas, O. et al. Mechanism of IS200/IS605 family DNA transposases: activation and transposon-directed target site selection. Cell 132, 208–220 (...
32 However, long non-coding RNA CEBPA-DT, a divergent transcript of CEBPA, has recently been found to promote liver cancer metastasis through DDR2/β-catenin activation.33,34 Germline SV-associated expression involving genes essential in cell lines To further sift through the germline SV-...
The Mechanism for Activation of GTP Hydrolysis on the Ribosome. Protein synthesis requires several guanosine triphosphatase (GTPase) factors, including elongation factor Tu (EF-Tu), which delivers aminoacyl-transfer RNA... Voorhees,M Rebecca,Schmeing,... - 《Science》 被引量: 304发表: 2010年 Inf...
None of these mutants are significantly altered in secondary structure, AdoMet or DNA affinity, kmethylation, kinactivation, or kcat. Although Val121 plays a critical role in both extrahelical base stabilization and catalysis, its position and mobility are not influenced by individual residues in ...
BMC Structural Biology BioMed Central Research article Open Access Natural history of S-adenosylmethionine-binding proteins Piotr Z Kozbial*1 and Arcady R Mushegian1,2 Address: 1Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110, USA and 2Department of Microbiology,...
Structural basis for the activation of the deubiquitinase calypso by the polycomb protein ASX. Structure 27, 528–536.e4 (2019). Article CAS PubMed Google Scholar Ge, W. et al. Basis of the H2AK119 specificity of the Polycomb repressive deubiquitinase. Nature 616, 176–182 (2023). ...
Here we argue that, on the basis of recent high-throughput genomic and proteomic screenings, DNA methylation can also have different outcomes, including activation of transcription. This is evidenced by the fact that transcription factors can interact with methylated DNA sequences. Furthermore, in ...