Katayama S., Skoog T., Jouhilahti E. M., et al. Gene expression analysis of skin grafts and cultured keratinocytes using synthetic RNA normalization reveals insights into differentiation and growth control. BMC Genomics. 2015;16:476
Analysis of human cortex nuclei included the following steps: (1) Loaded all 2842 cells. (2) Filtered out cells with less than 500 detected molecules (2306 cells retained). (3) Removed genes expressed in less than 20 cells, or more than 60% of cells. (4) Normalized each cell to total...