定量荧光聚合酶链反应(Q-PCR)和商用多重引物连接扩增(MLPA)试剂盒,再到基于低分辨全基因组测序的拷贝数变异测序(CNV-seq),流产物分析技术的分辨率越来越高,操作上更简单便捷,结果也变得越来越准确。
Consequently, analysis on STRs requires more sequencing depth with finely controlled target selection, but current target enrichment methods are not appropriate for this purpose. In this study, we developed a novel targeted sequencing technology, short tandem repeat sequencing (STR-Seq), by which ...
smart-seq2是按read计数,这里由于添加了UMI,是按molecule计数,也就是说,UMI的加入,确实减少了PCR扩增的偏差影响 另外图中看到,这里STRT-seq的spike-in方差一直要比内源基因的方差小,也就是说内源基因的变化幅度一直保持高位,体现了数据中包含多种细胞类型而导致的异质性,异质性导致了基因表达极度不均衡 6 降维 ...
文献解读 | 一种基于CNV-seq和STR-seq结合的检测引起自然流产的非整倍体及染色体异常来源方的新系统#文献解读# O文献解读 | 一种基于CNV-seq和STR-seq结合的... 文献解读 | 一种基于CNV-seq和STR-seq结合的检测引起自然流产的非整倍体及染色体异常来源方的新系统 在临床医学杂志 Journal ...
这篇文献使用的单细胞技术是STRT-seq,可以参考:单细胞转录组技术简史。 1. Human hematopoietic transcriptome reference Fig 1a:作者对21个正常人的外周血和骨髓进行了流式分选,得到了32个细胞类型,使用STRT-seq进行了单细胞测序。 Fig 1b:32种(7类)细胞的基因数 ...
STRT-Seq is a method similar to CEL-Seq that involves unique barcoding and sample pooling to overcome the challenges of samples with limited material(Islam et al., 2011)(Islam et al., 2012). In this method, single cells are first picked in individual tubes, where first-strand cDNA synthes...
Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting diluti...
低深度全基因组测序技术(CNV-seq)&(STR)背景我国出生缺陷现状2012年《出生缺陷防治报告》指出,我国出生缺陷发生率为5.6%。其中染色体畸变约占出生缺陷遗传学病因的80%以上,具体包括染色体数目异常、大片段缺失/重复及致病性基因组拷贝数变异(CopvNumber Variation ...
而STR分型方法是诊断染色体数目异常,对排除母源性细胞污染,以及染色体多倍体、单亲二倍体、性染色体数目异常的诊断具有快速、准确的特点,可弥补CNV-seq的不足。 CNV-seq联合STR分型方法应用于流产物染色体异常的检测,可覆盖更大范围的染...
A.《低深度全基因组测序技术在产前诊断的应用专家共识》建议将CNV-Seq技术与STR检测进行联合应用B.产前诊断中,胎儿细胞有受到母体细胞污染的可能C.流产物检测中,STR检测可使流产绒毛染色体检测的结果更准确D.产前筛查中,STR检测可确定样本中是否存在胎儿DNA此题..