Of these, 4,590 genes were expressed in 10% of ROI and 1,127 genes were expressed in 50% of ROI.#Conclusion:Significant transcriptional activity is seen within cerebral thrombus and preliminary analysis shows feasibility for a novel application of spatial transcriptomics. Further evaluation of ...
et al. Combined single-cell and spatial transcriptomics reveal the molecular, cellular and spatial bone marrow niche organization. Nat. Cell Biol. 22, 38–48 (2020). Article CAS PubMed Google Scholar Kim, H. et al. Development of a validated interferon score using NanoString technology. J....
Single-cell and Spatial Transcriptomics Reveals Ferroptosis as The Most Enriched Programmed Cell Death Process in Hemorrhage Stroke-induced Oligodendrocyte-mediated White Matter Injury. Int J Biol Sci 2024; 20(10):3842-3862. doi:10.7150/ijbs.96262. https://www.ijbs.com/v20p3842.htm Copy Other ...
Emerging understanding of brain plasticity has opened new avenues for the treatment of stroke. The promising preclinical evidence with neuroprotective drugs has not been confirmed in clinical trials, thus nowadays, researchers, pharmaceutical companies, and funding bodies hesitate to initiate these expensive...
Abstract Neuroglia critically shape the brain´s response to ischemic stroke. However, their phenotypic heterogeneity impedes a holistic understanding of the cellular composition of the early ischemic lesion. Here we present a single cell resolution transcriptomics dataset of the brain´s acute respons...
Single-cell transcriptomics characterizes cell types in the subventricular zone and uncovers molecular defects impairing adult neurogenesis. Cell Rep 2018, 25: 2457-2469.e8. Article CAS PubMed Google Scholar Zhu Y, Crowley SC, Latimer AJ, Lewis GM, Nash R, Kucenas S. Migratory neural crest ...
Secondly, we performed metabolomics, transcriptomics, and proteomics analysis on cerebral cortex. This multi-omics investigation unveiled noteworthy alterations in proteins and metabolites within pathways associated with neurotransmitter systems, heatstroke-induced mitochondrial dysfunction, encompassing histidine and...
Here, we describe a platform for spatially resolved, single-cell transcriptomics and proteomics, called tDISCO (tissue-digital microfluidic isolation of single cells for -Omics). We use tDISCO alongside two high-throughput platforms for spatial (Visium) and single-cell trans...
PageRank-based identification of signaling crosstalk from transcriptomics data: the case of Arabidopsis thaliana. Mol Biosyst 8, 1121–1127 (2012). 37. Winter, C. et al. Google goes cancer: improving outcome prediction for cancer patients by network-based ranking of marker genes. PLoS Comput ...
(Supp. Fig.6a–c). Future studies that combine calcium imaging with electrophysiology, single cell transcriptomics and/or post-mortem staining of proteins that associate with VIP neurons (calretinin, CCK, ChaT), would be needed unravel this mystery. Another potential limitation is our use of ...