Procedure for determining the genomic sequence comprising the steps of: - providing a physical map of a genome sample by sequencing the ends of fragments of clones defragmentos mixed artificial chromosomes; - providing a set of sequence reads sample genome; - generate a contig physical map and ...
Sequencing需要以下两种策略叠加: 1.Pooled genome sequence strategies :测同一物种的不同个体,不同个体叠加。 2.representative genome assembly approaches:因为有质量好的序列片段(reasonable N50 contig),所以可适用于缺乏长序列的情况。如果assembly质量好可以作为参考序列 Domestication:因为自然改变使得人改变,使得人为...
The advent of low cost next generation sequencing has made it possible to sequence a large number of dairy and beef bulls which can be used as a reference for imputation of whole genome sequence data. The aim of this study was to investigate the accuracy and speed of imputation from a high...
Next generation sequencing in cancer research and clinical application The wide application of next-generation sequencing (NGS), mainly through whole genome, exome and transcriptome sequencing, provides a high-resolution and g... D Shyr,L Qi - 《Biological Procedures Online》 被引量: 109发表: ...
genome sequencing (BSA-Seq) using contrasting bulks of drought-tolerant and drought-sensitive F1-clones. Applying QTLseqr, 15 QTLs comprising 588,983 single nucleotide polymorphisms (SNPs) in 2325 genes associated with drought stress tolerance were identified. SeqSNP analyses in an association panel ...
Lastly, as EMS may produce multiple hits in the gene of interest[20], verifying the mutation responsible for the scored phenotype requires laborious complementation tests or full-genome sequencing. The time and effort has been considerably reduced nowadays in order to plot the molecular nature of ...
A consortium of seven institutions in New York City enrolled 30 patients with glioblastoma and performed tumor whole genome sequencing (WGS) and RNA sequencing (RNA-seq; collectively WGS/RNA-seq); 20 of these patients were also analyzed with independent targeted panel sequencing. We also compared ...
We survey here a wide range of emerging sequencing platforms and analytical tools for de novo assembly, provide background information for each of their steps, and discuss the spectrum of available options. Our decision tree recommends workflows for the generation of a high-quality genome assembly ...
The current trajectory for crop yields is insufficient to nourish the world’s population by 20501. Greater and more consistent crop production must be achieved against a backdrop of climatic stress that limits yields, owing to shifts in pests and pathogens, precipitation, heat-waves and other we...
Gene editing technology from the recently developed engineered nucleases such as TALENs has been successfully employed in genome modification of a variety of species, and will be applied in gene therapy of selected human diseases. The genetic basis of DMD and BMD indicates that DMD is a good ...