网络肿瘤坏死因子受体 网络释义 1. 肿瘤坏死因子受体 肿瘤坏死因子受体(sTNFRII)在大肠杆菌中高效表达及纯化酵母活性物质(1)提取最佳条件的研究酵母活性物质(2制备工艺的研 … www.lwtxw.com|基于 1 个网页
构建了人肿瘤坏死因子受体II胞外区、人脂联素球部与人IgG1 Fc的融合基因sTNFRII-gAD-Fc的真核表达载体,在CHO-S细胞中快速高效表达,探索了无血清悬浮流加培养工艺。应用重组PCR将人IgG1 Fc基因片段与sTNFRII-gAD基因片段融合,构建pMH3-sTNFRII-gAD-Fc表达载体,电转染至CHO-S细胞,挑选G418抗性的高表达单克隆,...
成功构建pMH3- sTNFRII-gAD-Fc表达载体,检测到sTNFRII-gAD-Fc在CHO-S细胞培养上清中以二聚体和多聚体形式表达,获得了高表达(75 μg/mL)单克隆细胞株,且该蛋白具有显著抑制TNFα杀伤L929细胞的活性.在摇瓶中无血清批次培养和转瓶及生物反应器中流加培养时产量分别为10.0 mg/L,18.3 mg/L和20.5 mg/L.利用...
产品名称:Human sTNFRII ELISA Kit 我公司总代理,华东地区代理Human sTNFRII ELISA Kit专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等.总代理,华东地区代理Human sTNFRII ELISA Kit产品涉及分子生物...
The value of soluble receptor for tumor necrosis factor type II (sTNFRII) as a strong and early predictor of HIV disease progression was suggested. Recently it has been reported that sTNFRII may provide an indication of the HIV load. In this work we focused on the relationship between sTNFRI...
本研究设计和构建了一种人肿瘤坏死因子受体II胞外区与人脂联素球部的融合基因sTNFRII-gAD,且相应的融合蛋白在哺乳动物细胞BHK-21S的无血清培养体系中实现了表达,并对该融合蛋白进行了初步鉴定.首先,用RT-PCR方法从人的外周血淋巴细胞总RNA中扩增人肿瘤坏死因子II型受体胞外区基因片段,与脂联素球部基因片段融合,克隆...
Here, we constructed a strain of sTNFRⅡ-Fc-expressing MSCs (sTNFRⅡ-MSC), aiming to offset the deficiency of those two interventions. Methods Constructed sTNFRII-Fc lentiviral vector was used to infect human umbilical cord-derived MSCs, and sTNFRII-MSC stable cell line was generated by ...
Soluble tumor necrosis factor receptor type II (sTNFRII) in HIV-infected patients: relationship with the plasma level of HIV-1 - Hober, Benyoucef, et al. - 1999 () Citation Context ..., since sTNFRII acts as a “scavenger” for TNF-. The balance of TNF- and sTNFRII in the lung ...
Levels of the immuno-inflammatory markers were similar in both groups except sTNFRII >5,000 pg/mL (52% cases versus 24% controls; P = 0.001). No marker was associated with histological steatosis. Under multivariate analysis, baseline adiponectin and sTNFRII levels were independently ...
We have recently constructed a novel fusion protein sTNFRII-gAD, which is composed of a soluble TNF receptor II and a globular domain of adiponectin. Utilizing the inclination of gAD to form homologous trimer naturally, we sought to explore TNFα antagonism of the novel trimerized sTNFRII-gAD ...