In vertebrate cells and some insect cells, there are not only chemical modifications at both ends of the final mRNA product prior to its engagement with ribosomes in the cytoplasm but cleavage of the primary transcript and reunion of selected polynucleotide stretches鈥擱NA:RNA splicing鈥攊s ...
Here we show that the plant TREX-2 complex promotes multiple steps in miRNA biogenesis, including transcription, processing and nuclear export. THP1 and SAC3A—the core subunits of TREX-2—interact and colocalize with RNA polymerase II to promote the transcription of MIR genes in the nucleoplasm...
The polymerase chain reaction (PCR) is a process by which amplification of a targeted region of DNA occurs. It is performed in the thermocycler. The main components used in PCR reaction are- template DNA, dNTPs, primer, Taq DNA polymerase, and buffer....
The gel was directly scanned by Fujifilm FLA 9000 Imager (fluorescence detection mode), and analysed (see Data processing and analysis). 3′-adapter ligation reaction The reaction mixture consisted of 1 μl of 1 μM N40-3′OH RNA (0.1 μM final), 1 μl of 1, 2.5, 5 or ...
Ladder type: DNA or RNA Fragment structure: single-stranded or double-stranded Conformation: supercoiled, open circular, or linear to ensure appropriate comparisons of migration Number of DNA/RNA fragments and their separation patterns for...
Nucleic acids are large biomolecules used to store, transfer and convey genetic information in cells. Explore the structure of nucleotides and what polynucleotide and phosphodiester bonds do. Related to this Question Describe the steps for DNA processing. ...
However, it requires a large amount of sample input and involves extensive data processing, especially when analyzing the human genome, which is large and complex. Top b. De novo sequencing When genomic data for a particular o...
This paper emphasizes the class of activities in which a general purpose computer, complete with a library of basic programs, is further programmed to perform operations leading to ever higher-level information processing functions such as learning and problem solving. This informative article will be...
It is located in the large subunit of the ribosomes and therefore, the primary function of peptidyl transferase is to catalyze the addition of amino acid residues allowing the polypeptide chain to grow. The peptidyl transferase enzyme is entirely made up of RNA and its mechanism is mediated by...
Eukaryotic ribosomes consist of a small 40S and a large 60S subunit that are assembled in a highly coordinated manner. More than 200 factors ensure correct modification, processing and folding of ribosomal RNA and the timely incorporation of ribosomal proteins1,2. Small subunit maturation ends in ...