Unlike in metazoans, the stepwise biogenesis of microRNAs (miRNAs) in plants occurs within the nucleus. Whether or how the major steps in miRNA biogenesis are coordinated is largely unknown. Here we show that the plant TREX-2 complex promotes multiple st
We found higher density of TF binding sites in flanking regions of transcription start site (TSS), and almost no enrichment of TF binding sites in flanking regions of transcription terminal site (TTS) (Fig.2e), which suggests an uneven distribution of TF binding sites in gene and its flanking...
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In mammals, the majority of the genome is kept in a DNA methylated state, whereas the Polycomb repressive complexes regulate the unmethylated CpG-rich promoters of developmental genes. In contrast to this general framework, the extra-embryonic lineages display non-canonical, globally intermediate DNA ...
RNA extraction, transcriptome sequencing, and data analysis By analysing physiological traits, we screened the two materials with the greatest differences between 0 and 72 h of drought stress. Therefore, a transcriptome assay was performed using the leaves and roots of sunflower seedlings after 0 h...
Early detection of cancer significantly improves patient outcomes, with biomarkers offering a promising avenue for earlier and more precise diagnoses. Microfluidic biosensors have emerged as a powerful tool for detecting these biomarkers in body fluids,
Quantitative PCR (qPCR) was used to evaluate changes in expression levels of phytoene desaturase (PDS) upon VIGS infection. Total RNA extraction was performed as shown in the previous section. qPCR was carried out on the same RNA samples (9 biologically distinct samples), with each reaction repe...
The proposed scheme consists of a video preprocessing step plus four main steps: colored video components extraction and padding, frame components splitting, frame components scrambling, key generation, and diffusion step. The input-colored video is preprocessed to extract individual frames. The three ...
As a key component of the RNA-induced silencing complex (RISC), Argonaute2 (Ago2) exhibits a dual function regulatory role in tumor progression. However, the mechanistic basis of differential regulation remains elusive. p63 is a homolog of the tumor suppressor p53. p63 isoforms play a critical...
To further study the regulation of the development of adult firefly light organs, we focused on AlABD-B and AlUNC-4 as putatively key transcription factors. There are two key steps in the development of adult firefly light organs and luminescence. One is the expression of the luciferase gene...