室温离心SDS法快速提取苹果等植物组织 [17]Wang Y,LiZ,LiuXX,eta1.AComparativeStudyonThreeMethods RNA[J1.同艺学报,2006,33(3):470. fortheExtractionofTotalRNA from Pinustmngeana[J].Agricultural [4] 刘洋,何心尧,马红波,等.用CTAB-PVP法提取棉花各组织总 RNA Blotechnology,2011,12(5):663—665. 的...
In our experiments, we have demonstrated the utility of projections for the extraction of single-cell transcriptomes from diverse single-cell resolution assays such as spatial transcriptomes and Patch-seq. At its heart, projection maps RNA samples into the cell state space defined by a single-cell...
Keywords:RNAextraction;PVP;CTAB;heatingtime;Phenol/chloroform-isoamylalcohol 在生物及生物技术的研究和应用中,RNA的提取已经成为重要的基本实验技术。尽管已经建立了多种提取方法 [1,2] ,但对于特定的材料和不同的实验室,需要从材料,可利用的试剂及基金等方面考虑从而做出不同的选择。此外,提取的RNA的质量直接影响...
Absrtact :Taking tomato variety Moneymaker (MM )as the material ,compared the extraction effects of three methods including kit method ,Trizol method and Trizol improved method on total RNA from tomato leaves and fruits at different stages ,and measured the concentration ,purity and integrity ...
Previously, we introduced an advanced version of the single-step method for RNA extraction. We have now adapted this methodology for the isolation of total RNA, mRNA, small RNA and microRNA from whole blood, plasma or serum of human and animal origin. Th
The cDNA fragments were purified with the QiaQuick PCR extraction kit and end repaired, poly(A) was added, and the fragments were ligated to Illumina sequencing adapters. The size of the ligation products was detected using agarose gel electrophoresis, and the products were PCR amplified and ...
Methods Experimental design In the present study, we have tested a strategy to store lung tissues for the period of more than 2.5 yrs without any chemical treatment or processing, and investigated which RNA extraction technique (i.e. SGC and GTC) is preferable in terms of RNA recovery, purity...
*.fastq.gz: If--save_umi_intermedsis specified, FastQ filesafterUMI extraction will be placed in this directory. *.log: Log file generated by the UMI-toolsextractcommand. UMI-toolsdeduplicates reads based on unique molecular identifiers (UMIs) to address PCR-bias. Firstly, the UMI-toolsextrac...
(TIF). Fragment Analyzer electropherograms of total RNA obtained with different RNA extraction methods from 107 Candida cells, stored in TSB-G and RNAlater. (TIF 160 kb) Additional file 2: Table S1. RNA yield and quality for different RNA extraction methodsa. (PDF 178 kb) Additional file...
专利名称:METHOD OF DNA/RNA EXTRACTION USING SILYLATED QUATERNARY AMMONIUM COMPOUNDS (SIQAC) 发明人:COBB, Benjamin David,ROGERS, Jan 申请号:EP 14 71274 6.8 申请日:2014 0324 公开号:EP 297884 3A1 公开日:20160203 摘要:There is described a method of extracting DNA and/or RNA from a cell or ...