PCR (polymerase chain reaction) is a method to analyze a short sequence of DNA (or RNA) even in samples containing only minute quantities of DNA or RNA. PCR is used to reproduce (amplify) selected sections of DNA or RNA. Previously, amplification of DNA involved cloning the segments of int...
DNA Cloning | Definition, Process & Examples 5:31 Transgenic Organism | Definition, Examples & Scientific Interest 4:35 Embryonic & Adult Stem Cells | Overview & Features 6:53 DNA Fingerprinting | Definition, Uses & Steps 5:50 5:03 Next Lesson Ethical and Safety Concerns for DNA Tech...
Recombinant DNA is a segment of DNA that is produced by combining genetic material from at least two different species. Such new genetic combinations are of value to science, medicine, agriculture, and industry. Recombinant DNA technology was first devel
PCR is a technique used to amplify a million copies of a target DNA sequence. It mimics the replication process of the DNA that allows the cloning of sequences inside the cell. PCR is currently used in molecular biology, biotechnology, forensics, medical research, and agriculture. One...
The base excision repair (BER) pathway is responsible for repairing most endogenous base lesions and abnormal bases in the genome as well as similar lesions generated by several groups of environmental agents, or their metabolic intermediates. The BER pathway is also involved in repair of DNA ...
DNA Step 5Perform one-step RT-PCR Determine which RT-PCR procedure to use In performing RT-PCR, one-step and two-step methods are the two common approaches, each with its own advantages and disadvantages. One-step RT-PCR combines first-strand cDNA synthesis (RT) and ...
In addition to providing an excellent medium for fragment size analyses, agarose gels allow purification of DNA fragments. Since purification of DNA fragments size separated in an agarose gel is necessary for a number molecular techniques such as cloning, it is vital to be able to purify fragments...
Molecular cloning The DNA sequences that encode the two-subunit transketolase Rif15, and the separated subunits Rif15a and Rif15b were amplified from the genomic DNA ofAmycolatopsis mediterraneiU32 (A. mediterraneiU32 was deposited in Institute of Microbiology, Chinese Academy of Sciences designated ...
In search for genes involved in cell-cell interactions during hindbrain segmentation, we have screened for protein kinase genes with restricted expression patterns in this region of the CNS. We present the cloning of three...被引量: 197
In 2007, the father of cloning technology gave up his technology while the studies of embryonic stem cell have not yet stopped. But there exists a very serious ethical problem of embryonic stem cells in that a real embryonic stem cell has to be obtained from an organism, that is, to save...