GeneArt® Cell Lines and Proteins are a suite of service offerings designed to help facilitate protein expression in mammalian cell lines. We can help with every step of the process, including gene design, synthesis, cloning, protein production, and even...
Will the T7 promoter in mammalian expression vectors function in prokaryotes to promote expression of proteins from genes cloned into the multiple cloning site? What is the consensus Kozak sequence for mammalian expression and do I need to include a Koza...
Because of the great sensitivity, PCR has found popularity in a wide range of applications: DNA sequencing and gene reproduction & cloning. The cellular cloning is one of the most remarkable applications of PCR. It makes it possible to isolate, that is to say, to purify a gene without resor...
Describe the various techniques used in DNA cloning. Describe different steps of Sanger's method of nucleotides sequencing. Name the laboratory technique used to identify DNA. List the important features of DNA. List and describe the factors that can degrade DNA evidence. ...
1. Overview, types of genetic engineering, biosafety. (Homework: think of ideas for genetic engineering projects. Past projects have included more nutritious rice, super-sized fugu, therapy for cachexia in cancer, DNA origami, edible vaccines, human gene therapy, arsenic decontamination, allergy-free...
These steps typically involve DNA restriction, followed by vector ligation and transformation into an Escherichia coli host. Cloned DNA is replicated and amplified in vivo and purified for subsequent in vitro manipulations. How vectors and cloning strategies are chosen depends on experimental goals. ...
Describe the various techniques used in DNA cloning. Describe how to construct recombinant DNA molecules. Explain in simple terms the process of DNA replication. Explain fully how DNA is replicated. Describe DNA structure. Summarize the steps in DNA replication in prokaryotic cells. ...
These steps were repeated until the stain was gone. Then the protein was reduced with DTT and alkylated using iodoacetamide under standard conditions (35). Gel slices were put into 50 μl of acetonitrile for 10 min; after removal of the solvent, 20 μl of trypsin (0.2 mg/ml) was added...
First, fewer purification steps are required, which helps ensure a more quantitative recovery of the template and a better ability to normalize the results to the starting number of cells. Second, by avoiding any mRNA enrichment steps, o...
We can help with every step of the process, including gene design, synthesis, cloning, protein production, and even stable cell generation. Alternatively, we can help with just the parts of the process you request. Protein Expression and Production Servi...