Extraction was done 2 × 60 min with 30 mL methanol under stirring at RT. Extracts were filtered using folded filter paper (8–12 µm pore size) and dried using a rotary evaporator. The dried extract was dissolved in 3 mL methanol and analyzed using UPLC-HRMS. An UltiMate...
cells (GFP+ cells: 3.8%–9% in different donors) (Figures 1B and 1C) as well as levels of 2-LTR circles (Figure 1D, 10.8- to 39.8- fold; see Figure S1G for absolute 2-LTR copy numbers from individual donors) and of integrated viral DNA copies (Figure 1E, 9- to 138-fold). ...
DNA Extraction and Purification Protocol Required Reagents Lysis buffer: 1% SDS, 0.5 M NaCl Isopropanol 70% (v/v) ethanol Collect the cells for DNA extraction into 2 ml tubes. Note:If you are using cell suspensions, you can move directly to the lysis procedure. If you are using samples, ...
Unlike in metazoans, the stepwise biogenesis of microRNAs (miRNAs) in plants occurs within the nucleus. Whether or how the major steps in miRNA biogenesis are coordinated is largely unknown. Here we show that the plant TREX-2 complex promotes multiple st
Developmental cues to start meiosis occur late in plants. Ameiotic1 (Am1) encodes a plant-specific nuclear protein (AM1) required for meiotic entry and progression through early prophase I. Pollen mother cells (PMCs) remain mitotic in most am1 mutants in
Extractionof heavy metals can be achieved by recovering the precipitate of metals from microbial samples. Microorganism in Biodegradation and their Impact In nature, biodegradation is a natural process that is carried out to recycle wastes and break down complex and toxic organic compounds into a much...
Free radicals can cause DNA strand breaks in cells, which prevents them from proliferating. A lot of chronic disorders are caused by this. By consuming langsat fruit, we can prevent these other ailments brought on by free radicals that might be harmful to our health. ...
Cardinali G., Pellegrini L., Martini A. (1995). Improvement of chromosomal DNA extrac- tion from different yeast species by analysis of single preparation steps. Yeast, 11: 1027-1029.Improvement of chromosomal DNA extraction from different yeast species by analysis of single preparation steps - ...
DNA polymerase (TsingKe Biotech, Beijing, China). Plasmid isolations fromE. colicells were performed using the Plasmid Miniprep Kit (TsingKe Biotech, Beijing, China). Purification of DNA fragments from agarose gels or PCR reactions was carried out using Gel Extraction Kit (Omega, USA) and Cycle...
All proteins were tethered to polystyrene beads with a 1,010-bp DNA linker with the appropriate functionalizations. The 1,010-bp DNA linkers were created using PCR and the M13mp18 plasmid template. All primers were ordered from Integrated DNA Technologies (IDT). In the case of wtTrCel7A, ...