T cellsGLYCOLYSISLACTATESCD8 antigenLACTATIONLACTIC acidLactate is a key metabolite produced from glycolytic metabolism of glucose molecules, yet it also serves as a primary carbon fuel source for many cell types. In the tumor-immune microenvironment, effect of lactate on cancer and immune cells can...
Here we show that H9T, an engineered interleukin-2 partial agonist, promotes the expansion of CD8+ T cells without driving terminal differentiation. H9T led to altered STAT5 signalling and mediated distinctive downstream transcriptional, epigenetic and metabolic programs. In addition, H9T treatment ...
Stem cells are maintained by transcriptional programs that promote self-renewal and repress differentiation. Here, we found that the transcription factor c-Myb was essential for generating and maintaining stem cells in the CD8+ T cell memory compartment.
CAR+-T cells on day 10 post-activation. (Bottom) Percent of CD39+ cells in HA-CAR- vs. CAR+-T cells (n = 12 donors). (C) (Left) Representative flow cytometry analysis of CD39 expression in CD8+ vs. CD4+ HA-CAR-T cells 10 days post-activation. (Right) Percent of CD39+ ...
(Fig.11A; Supplementary Fig. 9F). Based on previous reports and marker gene expression, we classified the cells into eight distinct types: malignant cells, monocytes/macrophages, epithelial cells, plasma cells, endothelial cells, CD8 T cells, erythroblasts, and pericytes (Fig.11B; Supplementary ...
Furthermore, tumor cells induce pro-survival autophagy to prevent damage and increase their survival rate [12]. Wnt/β-catenin signaling, STAT3 signaling, PI3K/Akt and non-coding RNAs mainly demonstrate abnormal regulation in cancer and their targets have been of importance in tumor suppression [...
Apoptosis of HCC cells was detected by fow cytometry assay (J). K IFN-γ levels of CD8 + T cells were determined by fow cytometry analysis. NS: not signifcant, **p < 0.01, ***p < 0.001. Data from one representative experiment of three independent experiments are presented ...
of HCC cells. Additionally, the interaction between circ_0000972, miR-96-5p, and PFN1 was determined through bioinformatics analysis, dual-luciferase reporter assays, and rescue experiments. Circ_0000972 and PFN1 expression was significantly downregulated in HCC tissues and cells, while miR-96-5p ...
tumoricidal activity and increased levels of CD3+CD4+and CD3+CD8+T-cells in the tumor and tumor-draining lymph nodes (TDLNs) in the murine tumor model using 4T1 and MC38 cells. Conclusions The results show the first evidence of the essential role of the BRD4/nuclear PD-L1/RelB axis in...
Elimination of cancer stem cells (CSCs) and reinvigoration of antitumor immunity remain unmet challenges for cancer therapy. Tumor-associated macrophages (TAMs) constitute the prominant population of immune cells in tumor tissues, contributing to the for