CD34+ stem cell boost (SCB) can restore marrow function but may induce or exacerbate GvHD. We therefore investigated the feasibility and efficacy of CD34+-selected SCB in some patients with poor graft function. We present the results for eight patients (median age 46years) transplanted initially...
We cryopreserved NSCs for a 3-month period with CeA, SCB and the standard solution (Table 2). Results showed that cell viability decreased significantly after cryopreservation with all solutions when compared with fresh cells, with the exception of cells cryopreserved in CeA (one-way ANOVA: F(4...
Source: Springer Nature America, Inc Targeting Cancer Stem Cells with Natural Killer Cell Immunotherapy. Source: Informa UK Limited Cancer immunotherapy: the beginning of the end of cancer? Source: Springer Nature Prospects for chimeric antigen receptor (CAR) γδ T cells: A potential game changer...
The process of developing new and complex stem-cell-based therapeutics is incremental and requires decades of sustained collaboration among different stakeholders. In this Perspective, we address key ethical and policy challenges confronting the clinical translation of stem-cell-based interventions (SCBIs)...
Human Fertilisation and Embryology Authority (HFEA), Human Tissue Authority (HTA), Medicines and Healthcare Products Regulatory Agency (MHRA), Gene Therapy Advisory Committee (GTAC) and UK Stem Cell Bank Steering Committee (UKSCB). 195. Human Fertilisation and Embryology Authority, http://www.hfea...
Rate of inflammation SCB: Stem-CellBanker SD-OCT: Spectral domain optical coherence tomography SI: Scarring index αSMA: α-Smooth muscle actin TGF: Transforming growth factor TNC: Tenascin C TSG-6: Tumor necrosis factor α-stimulated gene 6 References...
The literature lacks high-quality randomized evidence, and there remains an unmet need for prospective studies to address the optimal dosing and manipulation of SCB. © 2021 American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc....
Megakaryocytes (MK) generate platelets. Recently, we and others, have reported MK also regulate hematopoietic stem cells (HSC). Here we show high ploidy large cytoplasmic megakaryocytes (LCM) are critical negative regulators of HSC and critical for platelet formation. Using a mouse knockout model...
CD34+-selected stem cell boost (SCB) without conditioning has recently been utilized for poor graft function (PGF) after allogeneic hematopoietic stem cell transplantation with promising results. Unfortunately, many patients have been unable to receive the boost infusion as their donors were unwilling ...
Cell clumps were harvested by using Accutase (Innovative Cell Technologies Inc.) and were gently dissociated into single cells. The cells were washed with a FACS buffer, which contained PBS, 0.1% NaN3, and 2% FBS. After being fixed and permeabilized with ice-cold 0.1% paraformaldehyde for 10 ...