据报道,PD-L1的上调导致有效的免疫抑制和肿瘤免疫逃逸,这解释了Setd2缺陷肿瘤发生免疫抑制的潜在原因。RT-qPCR还显示Setd2敲除细胞中MHC-I复合物核心基因H2K1的上调(图3E),细胞表面的H-2Kb /Db蛋白表达水平持续增加(图3F)。体内进一步证实了H2-K1的上调(图3B)。GSEA结果显示敲除Setd2促进MHC-I抗原加工和提呈...
IFN‐α upregulated antigen presentation related genes MHC I, TAP1, B2M, PSMB8, NLRC5 and transcriptional regulator STAT1, STAT2, IRF7 at a time and dose‐dependent manner. The silence of STAT1 or STAT2 both weakened IFN‐α‐induced increase of antigen presenting related molecules. IRF7 ...
STAT1 的肿瘤抑制作用可能与其在免 疫系统中的作用密切相关,STAT1 直接诱导转录激活子C ⅡTA 的表达,进而激活MHC Ⅱ [67] 190 的表达 。尽管STAT1 是肿瘤抑制因子,有迹象表明在某些情况下它也能促进肿瘤的生长。 有研究表明它可促进一种与IFN 信号无关的血液癌症的发生,并上调MHC Ⅰ的表达,表明 [68] STAT1 ...
The MHC class I-mediated antigen presentation pathway plays a critical role in antiviral immunity. Here we show that the MHC class I pathway is targeted by SARS-CoV-2. Analysis of the gene expression profile from COVID-19 patients as well as SARS-CoV-2 i
Values in b and c represent mean fluorescence intensity for MHC-I+/GFP+ cells. Mutant Stat1 Capacities for Dimerization, Tyrosine Phosphorylation, and DNA Binding—In view of the apparent absence of spontaneous function for mutant Stat1, we next conducted experiments to more directly monitor ...
Flow cytometry analysis of H-2Kb MHC Class I. SSM2 tumor cells were transduced with the two PrlR-specific shRNA hairpins. Surface expression of H-2Kb MHC class I was quantified using anti-H-2Kb-PE (BD Biosciences, San Jose, CA, USA). Immunoprecipitation and western blotting. SSM1, ...
IFNBCOL01 therapy dramatically improved the expression of Gzmb and MHC I in the tumor microenvironment [39]. This tumor-suppression effect may overcome the tumor promotion effect caused by the shut-down of IFN-I signaling in myeloid cells, leading to overall tumor inhibition. IFN-I transduces...
most efforts to increase therapeutic efficacy involve restoring STAT1 expression. For example, Liang et al. demonstrated the use of a proteasome inhibitor, bortezomib, to restore STAT1 expression in colorectal cancer, leading to an increase in major histocompatibility complex (MHC) I expression for sub...
Expression of the human major histocompatibility complex (MHC) class I genes has been shown previously to increase at the transcriptional level following exposure to interferon-γ (IFN-γ). In this report we have examined the molecular mechanisms involved in the IFN-γ-induced transcription of the...
review JAK-STAT 2:3, e24672; July/August/September 2013; © 2013 Landes Bioscience REVIEW Computational models of JAK1/2-STAT1 signaling Anna Gambin1,2,*, Agata Charzyńska3, Aleksandra Ellert-Miklaszewska4, and Mikołaj Rybiński2,5 1Institute of Informatics; University of Warsaw; Warsaw...