1995. Microcalorimetric study of the enzymatic hydrolysis of starch: an α-amylase catalyzed reaction. Anal Chim Acta 300:287-292.G. Salieri,G. Vinci,M.L. Antonelli.Microcalorimetric study of the enzymatic hydrolysis of starch: An α-amylase catalyzed reaction[J]. Analytica Chimica Acta .1995...
The increase of amylose content in the first two wk may be due to the catalytic reaction of debranching enzyme in endogenous amylase, which can hydrolyze α-1,6-glucosidic bond of amylopectin. During the later two wk, the decrease of amylose might be that the amylose is transformed from the...
Km value decreased from 3.784 g/L to 2.234 g/L,and the specific activity increased from 17.8 U/nmol to 32.0 U/nmol.The half life at 65 ℃ declined from 10 min of CN7A to 4 min of SBD-CN7A.The fusion of SBD with α-amylase CN7A maked the enzyme more suitable for raw starch ...
In this lab you will measure the reaction of amylase with starch (amylose) by testing for the presence of starch with iodine reagent. When iodine is added to a starch solution, a blue-black color is formed. If sufficient amylase is added to t...
Effects of alpha-amylase reaction mechanisms on analysis of resistant-starch contents[J] . Samuel A. Moore,Yongfeng Ai,Fengdan Chang,Jay-lin Jane... Moore,Samuel,A.,... - 《Carbohydrate Polymers Scientific & Technological Aspects of Industrially Important Polysaccharides》 被引量: 17发表: 2015...
Starch is a water-insoluble polyglucan synthesized inside the plastid stroma within plant cells, serving a crucial role in the carbon budget of the whole plant by acting as a short-term and long-term store of energy. The highly complex, hierarchical structure of the starch granule arises from...
-amylase, and hydrolysis of the filtrate with acid. Starch content is calculated from the amount of glucose present as determined with glucose oxidase. 关键词: glucose, alkali treatment, bonding agents, recovery, pulp and paper technology, chemical analysis, papermaking, xanthides, alpha-amylase, ...
Thinning reaction, in which the starch polysaccharides are attacked by α-amylase, shortening the chain and reducing viscosity. • Saccharification, which produces glucose from the shortened polysaccharides catalysed by the glucoamylase. • Isomerization, which converts glucose into fructose, catalysed ...
In this paper, we describe a novel approach for the degradation of starch using the catalytic behaviour of AgNPs in an enzyme catalysed reaction of starch hydrolysis by α-amylase. AgNPs were synthesized by soluble starch reducing silver nitrate to silver atoms. An increase of 4.7-fold in ...