colour reflects either the phenotype (yellow), species with increased abundance (green) or species with decreased abundance (red) in response to RS. The colour of the connecting line reflects either the positive correlation (red) or negative correlation (blue) between microbes and the phenotypes. ...
Positive effect: X (u) = Xi Xmin Xmax Xmin (4) Negative effect: X (u) = 1 Xi Xmin Xmax Xmin (5) Where X(u) is the function value of a parameter; Xi is the data value of the point to be analyzed; Xmax is the maximum value in the data column of a ...
Can you describe the test done to find out if a plant needs CO to make starch. A test tube contains both starch and the enzyme amylase. After 30 minutes, the test for starch is negative and the test for simple sugar is positive. Explain what happened. Why is iodine used to stain onio...
Various tests were performed on this isolate including catalase, methyl red test, voges鈥損roskauer, indole, citrate utilization and nitrate reduction test. Positive results were obtained for the former two tests and negative results for the latter tests. The presence of exoenzymes like gelatinase,...
Explain why a mixed culture cannot be used to inoculate a differential media such as the triple sugar iron agar test. If the cultures overgrow to OD600 of 1 or 2, can you simply dilute the culture with more LB? Why or why not? Why is it necessary to use m...
This Gram-positive bacteria is known for high-level protein synthesis and secretion [42]. For instance, they synthesize α-amylases, which catalyzes the breakdown of long-chain carbohydrates to yield soluble products, mainly short oligosaccharides and dextrin. These enzymes are used as catalysts in...
Among Gram-positive bacter- ia, B. breve and relatives of E. rectale have been shown to produce large extracellular amylases that include multiple catalytic and substrate-binding domains (Ramsay et al., 2006; Motherway et al., 2008). No research has been carried out so far on the ...
The different letters indicate significant differences (p < 0.05) determined by Duncan’s multiple range test. Fructooligosaccharide (FOS) was used as a positive control. 3.6. Changes in intestinal microbiota by RBR To determine the effect of RBR extract on the intestinal environment, the ratio ...
Starch Hydrolysis test a. Media: Starch Agar b. Exoenzyme: Amylase C. Reagent: Iodine will turn dark blue/ black color in presence of starch D. Positive results= no blue/black around bacteria means the bacteria did produce amylase Negative results = blue/black around bacteria means the bacteri...
solution (0.1N iodine from VWR Cat. No. VW3207-1 diluted 4×) is added to the pellet and mixed. A positive (+) starch shows a range of color from blue to purple and the intensity of color is directly proportional to the concentration of starch. A negative result (−) remains ...