After spread plating a given sample on the agar plates, the typical yellow colonies of Xanthomonas are seen after incubating the plates at 26 ± 2 °C for 48 h. The starch hydrolysis is visualized as the zone of clearance around the colonies. In the absence of dyes, iodine solution (1%)...
(new streaks for isolation) Starch Agar Plate Inoculation method: surface streak with loop Contains: the starches amylopectin and amylose in agar to solidify Additional reagents added: iodine, combines with starch to produce a blue-black precipitate Discriminates organisms that can produce amylase to ...
In the starch hydrolysis test, the test bacteria are grown on agar plates containing starch. After colonies of the bacteria are visible, the plates are flooded with iodine solution. If the bacteria have the ability to hydrolyse starch, its colonies hydrolyse the starch ...
Then, after centrifugation (12,000 × g, 10 min), the fermentation supernatant was inoculated onto a starch screening plate (w/v) containing 2% soluble starch and 1% agar using a microplate replicator. The starch screening plate was placed at 30 ℃ for 2 h and then sprayed with iodine ...
Y. Zhou, H. Liu, M. Zhang, and N. Xu, "A new method for measuring total antioxidant capacity in urine using the iodine starch agar based on agar diffusion," Current Analytical Chem- istry, vol. 11, pp. 1-6, 2016.Zhou, Y.; Xu, N.; Zhang, M.; Liu, H. A New Method for ...
An aliquot of 10 mL test starch solution was transferred into a 50 mL volumetric flask, and we added 5 mL of 0.1 N HCl followed by 0.5 mL of iodine reagent. The volume was made to 50 mL using distilled water, and absorbance was recorded at 625 nm in a UV–VIS spectrophotometer (Sys...
The leaves were subsequently put on MS medium with 1,6% glucose, 5 mg/l naphthyl acetic acid, 0,2 mg/l benzylaminopurine, 250 mg/l claforan, 50 mg/l kanamycin or 1 mg/l hygromycin B, and 0,80% Bacto Agar for callus induction. After a one-week incubation at 25° C. and 3000...
Consequently, transparent clear zones are formed around the colonies that hydrolyze starch while the rest of the plate show a dark blue coloration as iodine forms the colored complex with starch. Media: Starch agaris a simple nutritive medium with starch added. Beef extract and pancreatic digest ...
Qualitative screening of the library of SPs was performed by cultivating individual colonies in 96-well plates, and the AmyZ1 activity in the supernatant was determined using a plate experiment in which the supernatant was inoculated into 1 % agar plates containing 2 % soluble starch. Then, the...
Approximately 20,000 cells in late log phase (1 × 107 cells/mL) were plated on TAP agar plates and air-dried for 30 min before UV treatment. Subsequently, the cultures were exposed to 40,000 μJ cm-1 of monochromatic UV light with a wavelength of 254 nm (CL-1000 UV crosslinker; UV...