Functional characterization of transport proteins using conventional electrophysiology can be challenging, especially for low turnover transporters or transporters from bacteria and intracellular compartments. Solid-supported membrane (SSM)-based electrophysiology is a sensitive and cell-free assay technique for ...
This chapter focuses on how the SSM-based electrophysiology has contributed to unraveling the transport mechanism of two prominent members of the P-type ATPase family, that is, the sarcoplasmic reticulum Ca-ATPase and the human Cu-ATPase (ATP7A and ATP7B)....
Determination of assay parameters for SSM-based electrophysiology assaySupport Protocol 5: Assay with stable bufferAlternate Protocol 3: Assay with ion gradientSupport Protocol 6: Determination of membrane/liposome concentrationSupport Protocol 7: Determination of substrate dependency kMPROTOCOL GROUP 3: Deter...
Andre Bazzone aAlexander Krner b cMelanie Meincke aManan Bhatt aSrujan Dondapati bMaria Barthmes aStefan Kubick b d eNiels Fertig a
SSM-based electrophysiology of F267C NhaA.Dudu AlkobyAbraham RimonMaral BurdakMiyer PatinoRuizOctavian CălinescuKlaus FendlerEtana Padan
Niessen, K.V., Muschik, S., Langguth, F., Rappengluck, S., Seeger, T., Thiermann, H., Worek, F., 2016. Functional analysis of Torpedo californica nicotinic acetylcholine receptors in multiple activation states by SSM-based electrophysiology. Toxicol. Lett. 247, 1-10....